Cellular and molecular requirements for Polar PIN targeting and transcytosis in plants

Kleine-Vehna, Jürgen, Langowskia, Lukasz, Wisniewskab, Justyna, Dhonuksheb, Pankaj, Brewer, Philip B. and Friml, Jirí (2008) Cellular and molecular requirements for Polar PIN targeting and transcytosis in plants. Molecular Plant, 1 6: 1056-1066. doi:10.1093/mp/ssn062

Author Kleine-Vehna, Jürgen
Langowskia, Lukasz
Wisniewskab, Justyna
Dhonuksheb, Pankaj
Brewer, Philip B.
Friml, Jirí
Title Cellular and molecular requirements for Polar PIN targeting and transcytosis in plants
Journal name Molecular Plant   Check publisher's open access policy
ISSN 1674-2052
Publication date 2008
Sub-type Article (original research)
DOI 10.1093/mp/ssn062
Open Access Status
Volume 1
Issue 6
Start page 1056
End page 1066
Total pages 11
Place of publication Oxford, England
Publisher Oxford University Press
Language eng
Subject 07 Agricultural and Veterinary Sciences
0703 Crop and Pasture Production
Abstract The polar, sub-cellular localization of PIN auxin efflux carriers determines the direction of intercellular auxin flow, thus defining the spatial aspect of auxin signalling. Dynamic, transcytosis-like relocalizations of PIN proteins occur in response to external and internal signals, integrating these signals into changes in auxin distribution. Here, we examine the cellular and molecular mechanisms of polar PIN delivery and transcytosis. The mechanisms of the ARF–GEF-dependent polar targeting and transcytosis are well conserved and show little variations among diverse Arabidopsis ecotypes consistent with their fundamental importance in regulating plant development. At the cellular level, we refine previous findings on the role of the actin cytoskeleton in apical and basal PIN targeting, and identify a previously unknown role for microtubules, specifically in basal targeting. PIN protein delivery to different sides of the cell is mediated by ARF-dependent trafficking with a previously unknown complex level of distinct ARF–GEF vesicle trafficking regulators. Our data suggest that alternative recruitment of PIN proteins by these distinct pathways can account for cell type- and cargo-specific aspects of polar targeting, as well as for polarity changes in response to different signals. The resulting dynamic PIN positioning to different sides of cells defines a three-dimensional pattern of auxin fluxes within plant tissues.
Keyword Cytoskeleton
Protein targeting
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
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Created: Wed, 09 Dec 2009, 09:25:43 EST by Christine Ouslinis on behalf of Faculty of Science