Persistent fetal infection with bovine viral diarrhea virus differentially affects maternal blood cell signal transduction pathways

Smirnova, Natalia P., Ptitsyn, Andrey A., Austin, Kathleen J., Bielefeldt-Ohmann, Helle, Van Campen, Hana, Han, Hyungchul, Van Olphen, Alberto L. and Hansen, Thomas R. (2009) Persistent fetal infection with bovine viral diarrhea virus differentially affects maternal blood cell signal transduction pathways. Physiological Genomics, 36 3: 129-139. doi:10.1152/physiolgenomics.90276.2008


Author Smirnova, Natalia P.
Ptitsyn, Andrey A.
Austin, Kathleen J.
Bielefeldt-Ohmann, Helle
Van Campen, Hana
Han, Hyungchul
Van Olphen, Alberto L.
Hansen, Thomas R.
Title Persistent fetal infection with bovine viral diarrhea virus differentially affects maternal blood cell signal transduction pathways
Journal name Physiological Genomics   Check publisher's open access policy
ISSN 1094-8341
Publication date 2009-02
Sub-type Article (original research)
DOI 10.1152/physiolgenomics.90276.2008
Open Access Status
Volume 36
Issue 3
Start page 129
End page 139
Total pages 11
Place of publication Bethesda, U.S.
Publisher American Physiological Society
Language eng
Formatted abstract
The consequences of viral infection during pregnancy include impact on fetal and maternal immune responses and on fetal development. Transplacental infection in cattle with noncytopathic bovine viral diarrhea virus (ncpBVDV) during early gestation results in persistently infected (PI) fetuses with life-long viremia and susceptibility to infections. Infection of the fetus during the third trimester or after birth leads to a transient infection cleared by a competent immune system. We hypothesized that ncpBVDV infection and presence of an infected fetus would alter immune response and lead to downregulation of proinflammatory processes in pregnant dams. Naïve pregnant heifers were challenged with ncpBVDV2 on day 75 (PI fetus) and day 175 [transiently infected (TI) fetus] or kept uninfected (healthy control fetus). Maternal blood samples were collected up to day 190 of gestation. Genome-wide microarray analysis of gene expression in maternal peripheral white blood cells, performed on days 160 and 190 of gestation, revealed multiple signal transduction pathways affected by ncpBVDV infection. Acute infection and presence of a TI fetus caused upregulation of the type I interferon (IFN) pathway genes, including dsRNA sensors and IFN-stimulated genes. The presence of a PI fetus caused prolonged downregulation of chemokine receptor 4 (CXCR4) and T cell receptor (TCR) signaling in maternal blood cells. We conclude that: 1) infection with ncpBVDV induces a vigorous type I IFN response, and 2) presence of a PI fetus causes downregulation of important signaling pathways in the blood of the dam, which could have deleterious consequences on fetal development and the immune response.
Keyword Interferon
CXCL12/CXCR4
T cell receptor
Gene expression
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 09 Dec 2009, 09:20:14 EST by Rosalind Blair on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc