Transplacental infection with non-cytopathic bovine viral diarrhoea virus types 1b and 2: Viral spread and molecular neuropathology

Bielefeldt-Ohmann, H., Tolnay, A. E., Reisenhauer, C. E., Smirnova, N. and Van Campen, H. (2008) Transplacental infection with non-cytopathic bovine viral diarrhoea virus types 1b and 2: Viral spread and molecular neuropathology. Journal of Comparative Pathology, 138 2-3: 72-85. doi:10.1016/j.jcpa.2007.10.006


Author Bielefeldt-Ohmann, H.
Tolnay, A. E.
Reisenhauer, C. E.
Smirnova, N.
Van Campen, H.
Title Transplacental infection with non-cytopathic bovine viral diarrhoea virus types 1b and 2: Viral spread and molecular neuropathology
Journal name Journal of Comparative Pathology   Check publisher's open access policy
ISSN 0021-9975
Publication date 2008-02
Sub-type Article (original research)
DOI 10.1016/j.jcpa.2007.10.006
Open Access Status
Volume 138
Issue 2-3
Start page 72
End page 85
Total pages 14
Publisher Elsevier B.V.
Language eng
Subject 07 Agricultural and Veterinary Sciences
0707 Veterinary Sciences
Abstract Infection with bovine viral diarrhoea virus (BVDV) represents a reproducible natural animal model in which to study mechanisms of transplacental viral infection. In the present study, BVDV-seronegative heifers were challenged intranasally with non-cytopathic BVDV of genotype 1b or 2. Fetuses were retrieved by caesarean section 7-114 days post-challenge of the dam and subjected to virological, histopathological and immunohistochemistry(IHC) studies. Gross and histopathological changes were only seen in fetuses infected at gestational age 75-85 days and retrieved at gestational age 190 days. Viral antigen could be detected in most tissues from 14 days post-infection, but the primary target organs for histopathological changes were brain, liver and spleen. In the brain, microscopical changes included leucomalacia and macrophage infiltration of meninges and neuropil. Viral antigen was detected in neurons, oligodendrocyte precursors and infiltrating macrophages. IHC revealed normal to slightly increased expression of hypoxia-inducible factor-1alpha (HIF-1alpha) in the infected fetuses, with evidence of neuronal apoptosis and induction of inducible nitric oxide synthase (iNOS) and phospho-p38alpha mitogen-activated protein kinase (MAPK). These findings suggest that hypoxia may play only a limited role in the pathogenesis of the neural lesions. By contrast, virus-induced cytokine cascades, as part of the fetal innate immune response, and apoptosis of neurons and glial precursor cells may be central to the development of lesions.
Keyword Bovine Viral Diarrhoea Virus
Transplacental Infection
Hypoxia
Neural Lesions
Apoptosis
Bovine Fetus
Neuropathology
Congenital Infection
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 08 Dec 2009, 14:21:18 EST by Rosalind Blair on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc