A rapid in-vitro digestibility assay based on glucometry for investigating kinetics of starch digestion

Sopade, Peter A. and Gidley, Michael J. (2009) A rapid in-vitro digestibility assay based on glucometry for investigating kinetics of starch digestion. Starch, 61 5: 245-255. doi:10.1002/star.200800102


Author Sopade, Peter A.
Gidley, Michael J.
Title A rapid in-vitro digestibility assay based on glucometry for investigating kinetics of starch digestion
Formatted title
A rapid in-vitro digestibility assay based on glucometry for investigating kinetics of starch digestion
Journal name Starch   Check publisher's open access policy
ISSN 0038-9056
1521-379X
Publication date 2009-05
Sub-type Article (original research)
DOI 10.1002/star.200800102
Volume 61
Issue 5
Start page 245
End page 255
Total pages 11
Editor Barbara Elvers
Place of publication Weinheim, Germany
Publisher Wiley - VCH Verlag GmbH & Co.
Collection year 2010
Language eng
Formatted abstract
The potential of glucometry in in-vitro starch digestion was investigated for developing a rapid procedure to understand kinetics of digestion. A hand-held glucometer, intended for testing of plasma glucose levels, was used for the assay of glucose released by the combined action of α-amylase and amyloglucosidase on a range of starch substrates. The glucometer was sensitive to glucose concentrations in water, and its readings were independent of pH (7.7 and 3.9) and temperature (37°C and 25°C) of the glucose solution, but dependent on lactose and maltose concentrations. Neither fructose nor sucrose affected the readings. Digested starch calculated from the glucometer was not significantly (p > 0.05) different from that calculated from spectrophotometry. Particle size of substrate, sample formulation, grain genotype, and processing affected the glucometer readings as expected from how these factors influence starch digestibility. Corrections are required when samples containing lactose and maltose prior to in-vitro digestion analysis are studied. The developed rapid procedure can be used to collect large numbers of data points per sample per analysis for better understanding the kinetics of starch digestion, and increased confidence level in modelling the digestogram. The glucose detection method is robust and could be adapted for non-laboratory use. Single-point data can also be extracted from digestograms for comparative analysis.
Keyword Glucometer
Amylase
Starch digestibility
Digestogram
Blood glucose meter
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Part of this paper was presented at the 41st Annual Conference of the Australian Institute of Food Science and Technology, 21-24 July, 2008, Sydney, Australia.

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
ERA 2012 Admin Only
Centre for Nutrition and Food Sciences Publications
 
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Created: Tue, 08 Dec 2009, 14:08:22 EST by Emma Cushworth on behalf of Centre for Nutrition and Food Sciences