Association of orthodenticle with Natural Variation for Early Embryonic Patterning in Drosophila melanogaster

Goering, LM, Hunt, PK, Heighington, C, Busick, C, Pennings, PS, Hermisson, J, Kumar, S and Gibson, G (2009) Association of orthodenticle with Natural Variation for Early Embryonic Patterning in Drosophila melanogaster. Journal of Experimental Zoology Part B-Molecular and Developmental Evolution, 312B 8: 841-854. doi:10.1002/jez.b.21299


Author Goering, LM
Hunt, PK
Heighington, C
Busick, C
Pennings, PS
Hermisson, J
Kumar, S
Gibson, G
Title Association of orthodenticle with Natural Variation for Early Embryonic Patterning in Drosophila melanogaster
Formatted title
Association of orthodenticle with Natural Variation for Early Embryonic Patterning in Drosophila melanogaster
Journal name Journal of Experimental Zoology Part B-Molecular and Developmental Evolution   Check publisher's open access policy
ISSN 1552-5007
Publication date 2009-12
Year available 2009
Sub-type Article (original research)
DOI 10.1002/jez.b.21299
Volume 312B
Issue 8
Start page 841
End page 854
Total pages 14
Editor G. P. Wagner
Place of publication United States
Publisher John Wiley & Sons
Collection year 2010
Language eng
Subject 0604 Genetics
970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
C1
Formatted abstract
Although it is well established that cis-acting regulatory variation contributes to morphological evolution between species, few concrete examples of polymorphism affecting developmental patterning within species have been demonstrated. Early embryogenesis in Drosophila is initiated by a gradient of Bicoid morphogen activity that results in differential expression of multiple target genes. In a screen for genetic variation affecting this process, we surveyed 96 wild-type lines of Drosophila melanogaster for polymorphisms in binding sites within 16 Bicoid cis-regulatory response elements. One common polymorphism in the orthodenticle (otd) early head enhancer is associated with a complex series of indels/substitutions that define two distinct haplotypes. The middle region of this enhancer exhibits an unusual pattern of nucleotide diversity that does not easily fit into standard models of selection and demography. Population Gene Expression Maps, generated by extracting binary expression profiles from normalized embryo images, revealed a ventral reduction of otd transcript abundance in one of the haplotypes that was recapitulated in expression of transgenic constructs containing the two alleles. We thus demonstrate that even a process as robust as early developmental patterning is affected by standing genetic variation, intriguingly involving otd, whose morphogenetic function bicoid is thought to have displaced during dipteran evolution.
Keyword BICOID MORPHOGEN GRADIENT
GENE-EXPRESSION PATTERNS
POSITIVE SELECTION
REGULATORY MUTATIONS
EVOLUTION
DNA
SEGMENTATION
POLYMORPHISM
PROTEIN
BLASTODERM
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Sun, 06 Dec 2009, 00:05:35 EST