Integration of clinical data, pathology, and cDNA microarrays in influenza virus-infected pigtailed macaques (Macaca nemestri)

Baskin, Carole R., Garcia-Sastre, Adolfo, Tumpey, Terrence M., Bielefeldt-Ohmann, Helle, Carter, Victoria S., Nistal-Villan, Estanislao and Katzel, Michael G. (2004) Integration of clinical data, pathology, and cDNA microarrays in influenza virus-infected pigtailed macaques (Macaca nemestri). Journal of Virology, 78 19: 10420-10432. doi:10.1128/JVI.78.19.10420-10432.2004


Author Baskin, Carole R.
Garcia-Sastre, Adolfo
Tumpey, Terrence M.
Bielefeldt-Ohmann, Helle
Carter, Victoria S.
Nistal-Villan, Estanislao
Katzel, Michael G.
Title Integration of clinical data, pathology, and cDNA microarrays in influenza virus-infected pigtailed macaques (Macaca nemestri)
Formatted title
Integration of clinical data, pathology, and cDNA microarrays in influenza virus-infected pigtailed macaques (Macaca nemestri)
Journal name Journal of Virology   Check publisher's open access policy
ISSN 1098-5514
0022-538X
Publication date 2004-10-01
Year available 2004
Sub-type Article (original research)
DOI 10.1128/JVI.78.19.10420-10432.2004
Open Access Status DOI
Volume 78
Issue 19
Start page 10420
End page 10432
Total pages 13
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject 07 Agricultural and Veterinary Sciences
0707 Veterinary Sciences
0799 Other Agricultural and Veterinary Sciences
0702 Animal Production
Abstract For most severe viral pandemics such as influenza and AIDS, the exact contribution of individual viral genes to pathogenicity is still largely unknown. A necessary step toward that understanding is a systematic comparison of different influenza virus strains at the level of transcriptional regulation in the host as a whole and interpretation of these complex genetic changes in the context of multifactorial clinical outcomes and pathology. We conducted a study by infecting pigtailed macaques (Macaca nemestrina) with a genetically reconstructed strain of human influenza H1N1 A/Texas/36/91 virus and hypothesized not only that these animals would respond to the virus similarly to humans, but that gene expression patterns in the lungs and tracheobronchial lymph nodes would fit into a coherent and complete picture of the host-virus interactions during infection. The disease observed in infected macaques simulated uncomplicated influenza in humans. Clinical signs and an antibody response appeared with induction of interferon and B-cell activation pathways, respectively. Transcriptional activation of inflammatory cells and apoptotic pathways coincided with gross and histopathological signs of inflammation, with tissue damage and concurrent signs of repair. Additionally, cDNA microarrays offered new evidence of the importance of cytotoxic T cells and natural killer cells throughout infection. With this experiment, we confirmed the suitability of the nonhuman primate model in the quest for understanding the individual and joint contributions of viral genes to influenza virus pathogenesis by using cDNA microarray technology and a reverse genetics approach.
Keyword Influenza
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Veterinary Science Publications
 
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Created: Fri, 04 Dec 2009, 13:31:44 EST by Rosalind Blair on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc