Assessment of Chinese medicinal herb metabolite profiles by UPLC-MS-based methodology for the detection of aristolochic acids

Jacob, Shana Sunny, Smith, Norman William and Legido-Quigley, Cristina (2007) Assessment of Chinese medicinal herb metabolite profiles by UPLC-MS-based methodology for the detection of aristolochic acids. Journal of Separation Science, 30 8: 1200-1206.


Author Jacob, Shana Sunny
Smith, Norman William
Legido-Quigley, Cristina
Title Assessment of Chinese medicinal herb metabolite profiles by UPLC-MS-based methodology for the detection of aristolochic acids
Journal name Journal of Separation Science  (ERA 2012 Listed)    (ERA 2010 Rank B)   Check publisher's open access policy
Publication date 2007-05
Sub-type Article
DOI 10.1002/jssc.200600517
Volume number 30
Issue number 8
ISSN 1615-9306; 1615-9304
Start page 1200
End page 1206
Total pages 7
Place of publication Weinheim, Germany
Publisher Wiley-VCH
Language eng
Subject 110404 Traditional Chinese Medicine and Treatments
1101 Medical Biochemistry and Metabolomics
Abstract In this study, aristolochic acid in different herbal medicines containing a mixture of varying herb species was identified through fingerprint pattern similarities. Aristolochic acid I and II are nephrotoxic compounds naturally present in the Aristolochia plant species that are commonly used in Chinese herbal medicines. Twenty-four commercially available herbal formulations were extracted into an aqueous solution and injected into a UPLC-MS system. All the samples were analysed by multiple reaction monitoring (MRM) to check for the presence of aristolochic acids I and II. The same samples were then fingerprinted using two different gradient methods and the chromatograms deconvoluted into retention time (RT) and masses for the chemicals present taking concentration into account. Statistical analysis of this data revealed that samples were highly heterogeneous, and that the main differences between the preparations were concentrations of polar compounds. A model was constructed where the samples could be separated into two groups differentiated by the presence of the two forms of aristolochic acid.
Keyword Chemometrics
Fingerprinting
Herbal medicines
UPLC-MS
Q-Index Code C1

 
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Created: Fri, 04 Dec 2009, 12:56:02 EST by Michael Affleck on behalf of Aust Institute for Bioengineering & Nanotechnology