A specific cis-acting element regulates in vitro transcription of sex-dependent mouse steroid 16α-hydroxylase (C-P450 16α) gene

Yoshioka, Hidefumi, Lang, Matti, Wong, Garry and Negishi, Masahiko (1990) A specific cis-acting element regulates in vitro transcription of sex-dependent mouse steroid 16α-hydroxylase (C-P450 16α) gene. Journal of Biological Chemistry, 265 24: 14612-14617.

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Author Yoshioka, Hidefumi
Lang, Matti
Wong, Garry
Negishi, Masahiko
Title A specific cis-acting element regulates in vitro transcription of sex-dependent mouse steroid 16α-hydroxylase (C-P450 16α) gene
Formatted title
A specific cis-acting element regulates in vitro transcription of sex-dependent mouse steroid 16α-hydroxylase (C-P450 16α) gene
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 1990-08
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 265
Issue 24
Start page 14612
End page 14617
Total pages 6
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
The mouse steroid 16α-hydroxylase (C-P450(16α)) family consists of structually similar genes with very divergent expressions (Wong, G., Itakura, T., Kawajiri, K., Skow, L., and Negishi, M. (1989) J. Biol. Chem. 264, 2920-2927). Of five family members, the C-P45016α gene exhibits a male-specific expression whereas the P450cb gene is equally expressed in both males and females although the level of expression is much lower than that of the C-P45016α gene. By DNase I footprinting and in vitro transcription of the C-P45016α gene, we determined two cis-acting transcription elements. SDI (sex difference information) in the -84/-102 region from the transcription start site is one of the two cis-acting transcription elements, which is specific to the sex-specific C-P450(16α) gene and confers a high transcription level of this gene. We substituted the nucleotide(s) in the SDI sequence to the corresponding base(s) in the P450cb gene promoter and tested each substituted gene promoter by in vitro transcription. The results indicate that the function of SDI as the transcription element depends most critically on the nucleotides at positions -100 to -98. Another cis-acting transcription element, CTE (common transcription element), is located between -44 and -68 in both C-P45016α and P450cb genes. A column chromatography of nuclear extract indicates that the proteins bound to SDI and CTE are different. We speculate therefore that SDI is a specific and necessary element involved in the male-specific C-P45016α gene transcription. The sequence comparisons of the corresponding regions of SDI and CTE among the mouse and the closely related rat P450 genes suggest that SDI is a newly evolved cis-acting element in the sex-specific C-P45016α gene which arose through gene duplication and selective nucleotide substitution.
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Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
 
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Created: Mon, 30 Nov 2009, 15:09:20 EST