Mouse steroid 15α-hydroxylase gene family: Identification of type II P-45015α as coumarin 7-hydroxylase

Negishi, M., Lindberg, R., Burkhardt, B., Ichikawa, T., Honkakoski, P. and Lang, M. (1989) Mouse steroid 15α-hydroxylase gene family: Identification of type II P-45015α as coumarin 7-hydroxylase. Biochemistry, 28 10: 4169-4172. doi:10.1021/bi00436a007


Author Negishi, M.
Lindberg, R.
Burkhardt, B.
Ichikawa, T.
Honkakoski, P.
Lang, M.
Title Mouse steroid 15α-hydroxylase gene family: Identification of type II P-45015α as coumarin 7-hydroxylase
Formatted title
Mouse steroid 15α-hydroxylase gene family: Identification of type II P-45015α as coumarin 7-hydroxylase
Journal name Biochemistry   Check publisher's open access policy
ISSN 0006-2960
Publication date 1989-05
Sub-type Article (original research)
DOI 10.1021/bi00436a007
Volume 28
Issue 10
Start page 4169
End page 4172
Total pages 4
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract We identified type II P-45015α as mouse coumarin 7-hydroxylase (P-450coh). Unlike type I P-45015α, the other member within the mouse steroid 15α-hydroxylase gene family, type II catalyzed little steroid 15α-hydroxylase activity, yet structurally there were only 11 substitutions between type I and type II P-45015αs within their 494 amino acid residues (Lindberg et al., 1989), and the N-terminal sequence (21 residues) of P-450coh was identical with that of both P-45015αs. Induction by pyrazole of coumarin 7-hydroxylase activity correlated well with the increase of type II P-45015α mRNA in 129/J male and female mice. Pyrazole, on the other hand, was less in males or not effective in females in inducing the 15α-hydroxylase activity and type I P-45015α mRNA. Expression of type I and II in COS-1 cells revealed that the latter catalyzed coumarin 7-hydroxylase activity at 10 to ∼14 pmol min-1 (mg of cellular protein)-1. The former, on the other hand, had a high testosterone 15α-hydroxylase but little coumarin 7-hydroxylase activity. It was concluded, therefore, that type II P-45015α is the mouse coumarin 7-hydroxylase. Identification of type II as the P-450 specific to coumarin 7-hydroxylase activity and characterization of its cDNA and gene, therefore, were significant advances toward understanding the basis of genetic regulation of this activity in mice (known as Coh locus).
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
 
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Created: Mon, 30 Nov 2009, 15:07:00 EST