Structural and biotransformational membrane changes in the liver and intestine during chronic ethanol administration

Koivusaari, Ulla, Norling, Anja, Lang, Matti and Hietanen, Eino (1981) Structural and biotransformational membrane changes in the liver and intestine during chronic ethanol administration. Toxicology, 20 2-3: 173-183. doi:10.1016/0300-483X(81)90049-4


Author Koivusaari, Ulla
Norling, Anja
Lang, Matti
Hietanen, Eino
Title Structural and biotransformational membrane changes in the liver and intestine during chronic ethanol administration
Journal name Toxicology   Check publisher's open access policy
ISSN 0300-483X
1879-3185
Publication date 1981
Sub-type Article (original research)
DOI 10.1016/0300-483X(81)90049-4
Volume 20
Issue 2-3
Start page 173
End page 183
Total pages 11
Place of publication Shannon, Co. Clare Ireland
Publisher Elsevier Ireland
Language eng
Abstract The binding of a fluorescent probe 1-anilinonaphthalene-8-sulphonic acid (1,8-ANS) to liver microsomal membranes was markedly increased after chronic ethanol administration while the binding of a non-ionised probe phenylnaphthylamine (PNA) was not altered. The increase in 1,8-ANS binding is in accordance with the simultaneous increase of the ethoxycoumarin O-de-ethylase activity and cytochrome P-450 concentration. Also the intestinal ethyoxycoumarin O-de-ethylase activity and cytochrome P-450 concentration were increased. No changes in the aryl hydrocarbon hydroxylase or UDP-glucoronosyltransferase activities were found. The chronic ethanol administration increased the phospholipid amount in the liver microsomes and altered the fatty acid composition of microsomal phospholipids by decreasing the amount of oleic acid and increasing linoleic acid proportion. The data suggest that chronic ethanol administration may effect the biotransformation enzyme activities by changing the structural properties of the membranes as well as increasing the cytochrome P-450 concentration.
Keyword 1,8-ANS
1,8-anilinonaphthalene-8-sulphonic acid
PNA
Phenylnaphthylamine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
 
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Created: Mon, 30 Nov 2009, 15:03:38 EST