Dietary cholesterol-induced enhancement of hepatic biotransformation rate in male rats

Hietanen, E., Hanninen, O., Laitenen, M. and Lang, M. (1978) Dietary cholesterol-induced enhancement of hepatic biotransformation rate in male rats. Pharmacology, 17 3: 163-172. doi:10.1159/000136850

Author Hietanen, E.
Hanninen, O.
Laitenen, M.
Lang, M.
Title Dietary cholesterol-induced enhancement of hepatic biotransformation rate in male rats
Journal name Pharmacology   Check publisher's open access policy
ISSN 0031-7012
Publication date 1978
Sub-type Article (original research)
DOI 10.1159/000136850
Volume 17
Issue 3
Start page 163
End page 172
Total pages 10
Place of publication Basel, Switerland
Publisher S. Karger
Language eng
Abstract Male rats were fed a cholesterol-free diet for 5 weeks, followed by a 2% cholesterol diet for 4 weeks. Another group of rats was continuously fed a cholesterol-free diet. A third group was fed standard pellets during the whole experiment. Hepatic microsomal protein and cholesterol contents and drug-metabolizing enzyme activities were measured. The cholesterol-rich diet increased microsomal protein content and this increase disappeared after trypsin digestion of microsomal membranes. Microsomal cholesterol content was enhanced three-fold by cholesterol feeding. Cytochrome P-450 concentration, NADPH cytochrome and aryl hydrocarbon hydroxylase activities showed only minor changes following cholesterol feeding. The p-nitroanisole O-demethylase and ethoxycoumarin deethylase activities were doubled by cholesterol in comparison to cholesterol-free diet. Trypsin digestion activated the UDP-glucuronosyltransferase enzyme eight- to ten-fold on a protein basis. Trypsin treatment increased the cholesterol activation of UDP-glucuronosyltransferase when compared to the activity in native microsomes. The data suggest that dietary cholesterol regulates the cholesterol content of microsomal membranes. The activities of drug-metabolizing enzymes are also altered, possibly due to the compositional changes of the membranes.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
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Created: Mon, 30 Nov 2009, 15:02:12 EST