LIVE CELL IMAGING REVEALS THE HETEROGENEITY OF OLFACTORY ENSHEATHING CELLS: IMPLICATIONS FOR NEURAL THERAPIES

St John, J, Windus, L, Lineburg, K, Scott, S, Ekberg, J, Hansen, E, Claxton, C, Key, B and Mackay-Sim, A (2009). LIVE CELL IMAGING REVEALS THE HETEROGENEITY OF OLFACTORY ENSHEATHING CELLS: IMPLICATIONS FOR NEURAL THERAPIES. In: GLIA. 9th European Meeting on Glial Cells in Health and Disease, Paris FRANCE, (S151-S151). SEP 08-12, 2009.

Author St John, J
Windus, L
Lineburg, K
Scott, S
Ekberg, J
Hansen, E
Claxton, C
Key, B
Mackay-Sim, A
Title of paper LIVE CELL IMAGING REVEALS THE HETEROGENEITY OF OLFACTORY ENSHEATHING CELLS: IMPLICATIONS FOR NEURAL THERAPIES
Conference name 9th European Meeting on Glial Cells in Health and Disease
Conference location Paris FRANCE
Conference dates SEP 08-12, 2009
Proceedings title GLIA   Check publisher's open access policy
Journal name Glia   Check publisher's open access policy
Publisher Wiley-Liss
Publication Year 2009
Sub-type Published abstract
ISSN 0894-1491
Volume 57
Issue 13
Start page S151
End page S151
Total pages 1
Language eng
Abstract/Summary The therapeutic transplantation of olfactory ensheathing cells (OECs)is being trialled in humans for spinal injury regeneration and other neural disorders. However in humans and in animal models of these therapies, the results have been limited or inconsistent. One reason for the variation in results is that it is often assumed that OECs are a uniform population of cells that have the same characteristics, and are therefore uniformly suitable for neural regenerative therapies. We have now used time-lapse imaging of OEC interactions to examine in detail the behavioural characteristics of anatomical and developmental subpopulations of OECs. We have found that OECs are a heterogeneous population of cells with fundamental differences in their behaviour and migration. Not only were peripheral OECs significantly different from OECs derived from the olfactory bulb, but OECs from different anatomical regions of the olfactory bulb displayed vastly different characteristics. These behaviours were consistent with their proposed roles in vivo with OECs from the olfactory bulb display a mix of adhesion, repulsion or indifference following cell-cell contact. Consistent with our previous findings, lamellipodial waves along the shaft of the OEC processes are always associated with initiating cell-cell contact and mediating the resultant behaviours of both peripheral and central OECs. These results have important implications for the use of OECs in neural regeneration therapies as it is clear that OECs are not a uniform population of cells. Instead, these cells display distinct behavioural differences depending on their topographical location and age. These differences in cell origin and behaviour are likely to contribute to the variations in OEC transplantation outcomes.
Formatted Abstract/Summary
The therapeutic transplantation of olfactory ensheathing cells (OECs)
is being trialled in humans for spinal injury regeneration and other
neural disorders. However in humans and in animal models of these
therapies, the results have been limited or inconsistent. One reason
for the variation in results is that it is often assumed that OECs are
a uniform population of cells that have the same characteristics, and
are therefore uniformly suitable for neural regenerative therapies.
We have now used time-lapse imaging of OEC interactions to examine
in detail the behavioural characteristics of anatomical and developmental
subpopulations of OECs. We have found that OECs are a
heterogeneous population of cells with fundamental differences in
their behaviour and migration. Not only were peripheral OECs significantly
different from OECs derived from the olfactory bulb, but
OECs from different anatomical regions of the olfactory bulb displayed
vastly different characteristics. These behaviours were consistent
with their proposed roles in vivo with OECs from the olfactory
bulb display a mix of adhesion, repulsion or indifference following
cell-cell contact. Consistent with our previous findings, lamellipodial
waves along the shaft of the OEC processes are always associated
with initiating cell-cell contact and mediating the resultant behaviours
of both peripheral and central OECs. These results have important
implications for the use of OECs in neural regeneration therapies
as it is clear that OECs are not a uniform population of cells.
Instead, these cells display distinct behavioural differences depending
on their topographical location and age. These differences in cell origin
and behaviour are likely to contribute to the variations in OEC
transplantation outcomes.
Subjects 920111 Nervous System and Disorders
060105 Cell Neurochemistry
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Google Scholar Search Google Scholar
Created: Sun, 29 Nov 2009, 00:04:14 EST