Variation in Antiviral Protection Mediated by Different Wolbachia Strains in Drosophila simulans

Osborne, S.E., Leong, Y., O’Neill, S.L. and Johnson, K.N. (2009) Variation in Antiviral Protection Mediated by Different Wolbachia Strains in Drosophila simulans. PLoS Pathogens, 5 11: Article number e1000656. doi:10.1371/journal.ppat.1000656


Author Osborne, S.E.
Leong, Y.
O’Neill, S.L.
Johnson, K.N.
Title Variation in Antiviral Protection Mediated by Different Wolbachia Strains in Drosophila simulans
Formatted title
Variation in Antiviral Protection Mediated by Different Wolbachia Strains in Drosophila simulans
Journal name PLoS Pathogens   Check publisher's open access policy
ISSN 1553-7366
Publication date 2009-11
Year available 2009
Sub-type Article (original research)
DOI 10.1371/journal.ppat.1000656
Open Access Status DOI
Volume 5
Issue 11
Start page Article number e1000656
Total pages 9
Editor Kasturi Haldar
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2010
Language eng
Subject 060504 Microbial Ecology
060808 Invertebrate Biology
970106 Expanding Knowledge in the Biological Sciences
Formatted abstract
Drosophila C virus (DCV) is a natural pathogen of Drosophila and a useful model for studying antiviral defences. The Drosophila host is also commonly infected with the widespread endosymbiotic bacteria Wolbachia pipientis. When DCV coinfects Wolbachia-infected D. melanogaster, virus particles accumulate more slowly and virus induced mortality is substantially delayed. Considering that Wolbachia is estimated to infect up to two-thirds of all insect species, the observed protective effects of Wolbachia may extend to a range of both beneficial and pest insects, including insects that vector important viral diseases of humans, animals and plants. Currently, Wolbachia-mediated antiviral protection has only been described from a limited number of very closely related strains that infect D. melanogaster. We used D. simulans and its naturally occurring Wolbachia infections to test the generality of the Wolbachia-mediated antiviral protection. We generated paired D. simulans lines either uninfected or infected with five different Wolbachia strains. Each paired fly line was challenged with DCV and Flock House virus. Significant antiviral protection was seen for some but not all of the Wolbachia strain-fly line combinations tested. In some cases, protection from virus-induced mortality was associated with a delay in virus accumulation, but some Wolbachia-infected flies were tolerant to high titres of DCV. The Wolbachia strains that did protect occurred at comparatively high density within the flies and were most closely related to the D. melanogaster Wolbachia strain wMel. These results indicate that Wolbachia-mediated antiviral protection is not ubiquitous, a finding that is important for understanding the distribution of Wolbachia and virus in natural insect populations.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Tue, 24 Nov 2009, 15:01:37 EST by Hayley Ware on behalf of School of Biological Sciences