Dengue viral envelope protein and histidine protonation

Yennamalli, RM, Kobe, B, Mark, AE, Young, PR and Subbarao, N (2009). Dengue viral envelope protein and histidine protonation. In: Indian Journal of Virology. XVIII National Conference of Indian Virological Society, Post Graduate Institute of Medical Education and Research, Chandigarh, India., (29-29). 11–13 December 2008.

Author Yennamalli, RM
Kobe, B
Mark, AE
Young, PR
Subbarao, N
Title of paper Dengue viral envelope protein and histidine protonation
Conference name XVIII National Conference of Indian Virological Society
Conference location Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Conference dates 11–13 December 2008
Proceedings title Indian Journal of Virology   Check publisher's open access policy
Journal name Indian Journal of Virology   Check publisher's open access policy
Publisher Indian Virological Society
Publication Year 2009
Sub-type Published abstract
ISSN 0970-2822
0974-0120
Volume 20
Issue 1
Start page 29
End page 29
Total pages 1
Language eng
Formatted Abstract/Summary
In enveloped viruses, like flaviviruses, that enter cells via receptor-mediated endocytosis, fusion is initiated in low-pH of the endosomal vesicle. Upon appropriate triggering, the fusion protein interacts with the target membrane through a hydrophobic fusion peptide and undergoes a conformational change that drives the membrane fusion reaction1. The initial maturation of the virion and the later triggering of conformational changes that lead to membrane fusion require a pH range that suggests that the protonation of one or more histidine residues is critical in both processes2. In the current study we have studied the fusion trigger by performing molecular dynamics simulation of the envelope protein after insilico mutation of three histidines (His 244, His 261 and His 317) for 20 nanoseconds. Significant changes were seen in the salt-bridge behavior, Root mean square deviation and fluctuation and structural differences between the initial and end structures.
Subjects EX
Q-Index Code EX
Q-Index Status Provisional Code

Document type: Conference Paper
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Mon, 23 Nov 2009, 15:27:50 EST