Fetal cells participate over time in the response to specific types of murine maternal hepatic injury

Khosrotehrani, Kiarash, Reyes, R.R., Johnson, K.L., Freeman, R.B., Salomon, R.N., Peter, I., Stroh, Helene, Guegan, S. and Bianchi, Diana W. (2007) Fetal cells participate over time in the response to specific types of murine maternal hepatic injury. Human Reproduction, 22 3: 654-661. doi:10.1093/humrep/del426


Author Khosrotehrani, Kiarash
Reyes, R.R.
Johnson, K.L.
Freeman, R.B.
Salomon, R.N.
Peter, I.
Stroh, Helene
Guegan, S.
Bianchi, Diana W.
Title Fetal cells participate over time in the response to specific types of murine maternal hepatic injury
Journal name Human Reproduction   Check publisher's open access policy
ISSN 1460-2350
0268-1161
Publication date 2007-03
Sub-type Article (original research)
DOI 10.1093/humrep/del426
Volume 22
Issue 3
Start page 654
End page 661
Total pages 8
Place of publication Oxford
Publisher Oxford University Press
Language eng
Subject 111402 Obstetrics and Gynaecology
111601 Cell Physiology
Abstract BACKGROUND: In humans, fetal microchimeric cells transferred to maternal tissues during pregnancy can adopt a hepatocyte phenotype. Our objective was to determine whether fetal cells participate in the response to specific murine post-partum hepatic injuries. METHODS: Wild-type female mice were bred to males transgenic for the enhanced green fluorescent protein (GFP) (n = 42). Following delivery, we created models of chemical or surgical injury with carbon tetrachloride (CCl4) injection or by performing partial hepatectomy. Liver injury was assessed histologically. Fetal cells in maternal liver were detected and measured by real-time PCR amplification of the gfp transgene and by immunofluorescence using anti-GFP antibodies. RESULTS: PCR results showed that in chemical but not surgical injury, fetal GFP+ cells were detectable in maternal liver and spleen and that fetal cell presence was significantly increased over time following injury (4 versus 8 weeks, P = 0.006 for liver and P = 0.0006 for spleen). In some animals, following chemical injury, GFP+ cells were detected by immunofluorescence. CONCLUSIONS: The results of this preliminary study suggest that specific types of injury may elicit different fetal cell responses in maternal organs. There is a significant effect of time on fetal cell presence in liver and spleen. Furthermore, real-time PCR amplification is more sensitive than immunofluorescence for the detection of microchimeric fetal cells.
Keyword carbon tetrachloride
fetal cell microchimerism
partial hepatectomy
pregnancy
stem cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Centre for Clinical Research Publications
 
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Created: Tue, 17 Nov 2009, 12:28:11 EST