A novel grass pollen allergen mimotope identified by phage display peptide library inhibits allergen-human IgE antibody interaction

Suphioglu, Cenk, Schappi, George, Kenrick, Josephine, Levy, David, Davies, Janet M. and O'Hehir, Robyn E. (2001) A novel grass pollen allergen mimotope identified by phage display peptide library inhibits allergen-human IgE antibody interaction. FEBS Letters, 502 1-2: 46-52. doi:10.1016/S0014-5793(01)02661-8


Author Suphioglu, Cenk
Schappi, George
Kenrick, Josephine
Levy, David
Davies, Janet M.
O'Hehir, Robyn E.
Title A novel grass pollen allergen mimotope identified by phage display peptide library inhibits allergen-human IgE antibody interaction
Journal name FEBS Letters   Check publisher's open access policy
ISSN 0014-5793
1873-3468
Publication date 2001-07-27
Sub-type Article (original research)
DOI 10.1016/S0014-5793(01)02661-8
Volume 502
Issue 1-2
Start page 46
End page 52
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract The aim of this study was to investigate the molecular basis of human IgE–allergen interaction by screening a phage-displayed peptide library with an allergen-specific human IgE-mimicking monoclonal antibody (mAb). A mAb that reacted with major grass pollen allergens was successfully identified and shown to inhibit human IgE–allergen interaction. Biopanning of a phage-displayed random peptide library with this mAb yielded a 12 amino acid long mimotope. A synthetic peptide based on this 12-mer mimotope inhibited mAb and human IgE binding to grass pollen extracts. Our results indicate that such synthetic peptide mimotopes of allergens have potential as novel therapeutic agents.
Keyword Grass pollen
Allergen
Epitope
Mimotope
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 17 Nov 2009, 12:25:21 EST