Use of phage display technology to investigate allergen-antibody interactions

Davies, Janet M., O'Hehir, Robyn E. and Suphioglu, Cenk (2000) Use of phage display technology to investigate allergen-antibody interactions. Journal of Allergy and Clinical Immunology, 105 6: 1085-1092. doi:10.1067/mai.2000.107040


Author Davies, Janet M.
O'Hehir, Robyn E.
Suphioglu, Cenk
Title Use of phage display technology to investigate allergen-antibody interactions
Journal name Journal of Allergy and Clinical Immunology   Check publisher's open access policy
ISSN 0091-6749
1097-6825
Publication date 2000-06
Sub-type Article (original research)
DOI 10.1067/mai.2000.107040
Volume 105
Issue 6
Start page 1085
End page 1092
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Mosby
Language eng
Abstract Phage display is an advanced technology that can be used to characterize the interactions of antibody with antigen at the molecular level. It provides valuable data when applied to the investigation of IgE interaction with allergens. The aim of this rostrum article is to provide an explanation of the potential of phage display for increasing the understanding of allergen-IgE interaction, the discovery of diagnostic reagents, and the development of novel therapeutics for the treatment of allergic disease. The significance of initial studies that have applied phage display technology in allergy research will be highlighted. Phage display has been used to clone human IgE to timothy grass pollen allergen Phl p 5, to characterize the epitopes for murine and human antibodies to a birch pollen allergen Bet v 1, and to elucidate the epitopes of a murine mAb to the house dust mite allergen Der p 1. The technology has identified peptides that functionally mimic sites of human IgE constant domains and that were used to raise antiserum for blocking binding of IgE to the FcϵRI on basophils and subsequent release of histamine. Phage display has also been used to characterize novel peanut and fungal allergens. The method has been used to increase our understanding of the molecular basis of allergen-IgE interactions and to develop clinically relevant reagents with the pharmacologic potential to block the effector phase of allergic reactions. Many advances from these early studies are likely as phage display technology evolves and allergists gain expertise in its research applications.
Keyword Phage display
IgE
FAb
Allergen
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 17 Nov 2009, 12:24:15 EST