Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences

Uludag, Yildiz, Li, Xin, Coleman, Heather, Efstathiou, Stacey and Cooper, Matthew A. (2008) Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences. The Analyst, 133 1: 52-57. doi:10.1039/b711850c

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Author Uludag, Yildiz
Li, Xin
Coleman, Heather
Efstathiou, Stacey
Cooper, Matthew A.
Title Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences
Journal name The Analyst   Check publisher's open access policy
ISSN 0003-2654
Publication date 2008-01
Sub-type Article (original research)
DOI 10.1039/b711850c
Open Access Status File (Publisher version)
Volume 133
Issue 1
Start page 52
End page 57
Total pages 6
Publisher Royal Society of Chemistry Publishing
Language eng
Subject 06 Biological Sciences
Abstract We describe the detection of specific, conserved DNA sequences of herpes simplex virus (HSV) type 1 by means of a novel, high sensitivity acoustic biosensor. Repeated assays on planar and polymeric carboxylic acid- and biotin-presenting surface chemistries enabled statistical comparison of assay specificity and sensitivity and evaluation of assay Z-factor scores. Using a three minute hybridisation with NeutrAvidin capture for signal enhancement, it was possible to detect HSV viral nucleic acids at 5.2 6 10211 M concentration. c The Royal Society of Chemistry 2008
Keyword Quartz-crystal-microbalance
Single-base mismatches
Amplified detection
Biosensor
gold
Nanoparticles
ICP0
VP16
PCR
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
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Created: Tue, 17 Nov 2009, 12:18:19 EST