Rab-subfamily-specific regions of Ypt7p are structurally different from other RabGTPases

Constantinescu, Alexandru-Tudor, Rak, Alexey, Alexandrov, Kirill, Esters, Heike, Goody, Roger S. and Scheldig, Axel J. (2002) Rab-subfamily-specific regions of Ypt7p are structurally different from other RabGTPases. Structure, 10 4: 569-579. doi:10.1016/S0969-2126(02)00737-2


Author Constantinescu, Alexandru-Tudor
Rak, Alexey
Alexandrov, Kirill
Esters, Heike
Goody, Roger S.
Scheldig, Axel J.
Title Rab-subfamily-specific regions of Ypt7p are structurally different from other RabGTPases
Journal name Structure   Check publisher's open access policy
ISSN 0969-2126
1878-4186
Publication date 2002-04
Sub-type Article (original research)
DOI 10.1016/S0969-2126(02)00737-2
Volume 10
Issue 4
Start page 569
End page 579
Total pages 11
Place of publication Cambridge, MA, United States
Publisher Cell Press
Language eng
Formatted abstract
The GTPase Ypt7p from S. cerevisiae is involved in late endosome-to-vacuole transport and homotypic vacuole fusion. We present crystal structures of the GDP- and GppNHp-bound conformation of Ypt7p solved at 1.35 and 1.6 Å resolution, respectively. Despite the similarity of the overall structure to other Ypt/Rab proteins, Ypt7p displays small but significant differences. The Ypt7p-specific residues Tyr33 and Tyr37 cause a difference in the main chain trace of the RabSF2 region and form a characteristic surface epitope. Ypt7p•GppNHp does not display the helix α2, characteristic of the Ras-superfamily, but instead possess an extended loop L4/L5. Due to insertions in loops L3 and L7, the neighboring RabSF1 and RabSF4 regions are different in their conformations to those of other Ypt/Rab proteins.
Keyword Crystal structure
GTP-binding protein
Vesicular transport
Ypt7p
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Institute for Molecular Bioscience - Publications
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 17 Nov 2009, 12:07:37 EST