Non-pathogenic trypanosomatid protozoa as a platform for protein research and production

Breitling, Reinhard, Klingner, Susanne, Callewaert, Nico, Pietrucha, Regina, Geyer, Anett, Ehrlich, Gunter, Hartung, Regina, Muller, Angelika, Contreras, Roland, Beverley, Stephen M. and Alexandrov, Kirill (2002) Non-pathogenic trypanosomatid protozoa as a platform for protein research and production. Protein Expression and Purification, 25 2: 209-218. doi:10.1016/S1046-5928(02)00001-3

Author Breitling, Reinhard
Klingner, Susanne
Callewaert, Nico
Pietrucha, Regina
Geyer, Anett
Ehrlich, Gunter
Hartung, Regina
Muller, Angelika
Contreras, Roland
Beverley, Stephen M.
Alexandrov, Kirill
Title Non-pathogenic trypanosomatid protozoa as a platform for protein research and production
Journal name Protein Expression and Purification   Check publisher's open access policy
ISSN 1046-5928
Publication date 2002-07
Sub-type Article (original research)
DOI 10.1016/S1046-5928(02)00001-3
Volume 25
Issue 2
Start page 209
End page 218
Total pages 10
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Formatted abstract
All currently existing eukaryotic protein expression systems are based on autonomous life forms. To exploit the potential practical benefits associated with parasitic organisms we have developed a new protein expression system based on Leishmania tarentolae (Trypanosomatidae), a protozoan parasite of lizards. To achieve strong transcription, the genes of interest were integrated into the small subunit ribosomal RNA gene. Expression levels obtained were up to 30 mg of recombinant protein per liter of suspension culture and increased linearly with the number of integrated gene copies. To assess the system's potential for production of post-translationally modified proteins, we have expressed human erythropoietin in L. tarentolae. The recombinant protein isolated from the culture supernatants was biologically active, natively processed at the N-terminus, and N-glycosylated. The N-glycosylation was exceptionally homogenous, with a mammalian-type biantennary oligosaccharide and the Man3GlcNAc2 core structure accounting for >90% of the glycans present. L. tarentolae is thus the first described biotechnologically useful unicellular eukaryotic organism producing biantennary fully galactosylated, core-α-1,6-fucosylated N-glycans.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Institute for Molecular Bioscience - Publications
Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Tue, 17 Nov 2009, 12:07:23 EST