An in vitro cultured rumen inoculum improves nitrogen digestion in mulga-fed sheep

Miller, S. M., Klieve, A. V., Plumb, J. J., Aisthorpe, R. and Blackall, L. L. (1997) An in vitro cultured rumen inoculum improves nitrogen digestion in mulga-fed sheep. Australian Journal of Agricultural Research, 48 4: 403-409. doi:10.1071/A96100

Author Miller, S. M.
Klieve, A. V.
Plumb, J. J.
Aisthorpe, R.
Blackall, L. L.
Title An in vitro cultured rumen inoculum improves nitrogen digestion in mulga-fed sheep
Journal name Australian Journal of Agricultural Research   Check publisher's open access policy
ISSN 0004-9409
Publication date 1997
Sub-type Article (original research)
DOI 10.1071/A96100
Volume 48
Issue 4
Start page 403
End page 409
Total pages 7
Place of publication Melbourne, Australia
Publisher CSIRO
Language eng
Subject 0605 Microbiology
07 Agricultural and Veterinary Sciences
Abstract Mixed cultures of anaerobic micro-organisms were derived from feral goat rumen fluid (FGRF) using a laboratory fermentor to selectively culture microbes actively degrading mulga, and were evaluated as rumen inocula in digestion and liveweight studies with mulga-fed sheep. When placed in the rumen of sheep, FGRF enhances mulga digestion; however, limited supplies of feral goats, the labour involved in locating and mustering goats, and likely variations in the microbial composition of FGRF between animals and localities make the production of an in vitro cultured inoculum a desirable alternative to enable widespread adoption. The cultured inoculum significantly (P < 0·05) improved nitrogen digestion and retention in mulga-fed sheep by 16 and 76%, respectively. Inocula consisting of simplified mixtures of bacteria isolated from sheep, feral goats, and native marsupials did not affect mulga digestion. In the first of 2 liveweight studies, sheep inoculated with the fermentor inoculum lost significantly less weight than uninoculated sheep for the first 57 days (0·3 v. 4·6 kg); however, after 83 days the difference in the rate of liveweight loss between the fermentor inoculum group and the uninoculated sheep was not significant (53 v. 95 g/day). In the second study, liveweight loss was not significantly reduced by the fermentor inoculum. An inoculum based on FGRF, and produced in vitro using a fermentor, is potentially valuable to grazing enterprises reliant on mulga-fed sheep. However, problems in generating a consistent inoculum need to be addressed before such an inoculum can be generally considered.
Keyword Tannins
Acacia aneura
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 17 Nov 2009, 12:02:16 EST