Ammonia-hyperproducing bacteria from New Zealand ruminants

Attwood, Graeme T., Klieve, Athol V., Ouwerkerk, Diane and Patel, Bharat K. C. (1998) Ammonia-hyperproducing bacteria from New Zealand ruminants. Applied and Environmental Microbiology, 64 5: 1796-1804.


Author Attwood, Graeme T.
Klieve, Athol V.
Ouwerkerk, Diane
Patel, Bharat K. C.
Title Ammonia-hyperproducing bacteria from New Zealand ruminants
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 1998-05
Sub-type Article (original research)
Volume 64
Issue 5
Start page 1796
End page 1804
Total pages 9
Place of publication Washington, D.C.
Publisher American Society for Microbiology
Language eng
Subject 07 Agricultural and Veterinary Sciences
0605 Microbiology
Abstract Pasture-grazed dairy cows, deer, and sheep were tested for the presence of ammonia-hyperproducing (HAP) bacteria in roll tubes containing a medium in which tryptone and Casamino Acids were the sole nitrogen and energy sources. Colonies able to grow on this medium represented 5.2, 1.3, and 11.6% of the total bacterial counts of dairy cows, deer, and sheep, respectively. A total of 14 morphologically distinct colonies were purified and studied further. Restriction fragment length polymorphisms of 16S rRNA genes indicated that all isolates differed from the previously described HAP bacteria, Clostridium aminophilum, Clostridium sticklandii, and Peptostreptococcus anaerobius. Carbon source utilization experiments showed that five isolates (C2, D1, D4, D5, and S1) were unable to use any, or very few, of the carbon sources tested. Biochemical tests and phylogenetic analyses of 16S ribosomal DNA sequences indicated that all isolates were monensin sensitive; that D1 and S1 belonged to the genus Peptostreptococcus, that D4 and D5 belonged to the family Bacteroidaceae, where D4 was similar to Fusobacterium necrophorum; and that C2 was most similar to an unidentified species from the genus Eubacterium. Growth on liquid medium containing tryptone and Casamino Acids as the sole nitrogen and energy source showed that D1, D4, and S1 grew rapidly (specific growth rates of 0.40, 0.35, and 0.29 h-1, respectively), while C2 and D5 were slow growers (0.25 and 0.10 h-1, respectively). Ammonia production rates were highest in D1 and D4, which produced 945.5 and 748.3 nmol/min per mg of protein, respectively. Tests of individual nitrogen sources indicated that D1 and D4 grew best on tryptone, S1 grew equally well on Casamino Acids or tryptone, and C2 and D5 grew poorly on all nitrogen sources. The intact proteins casein and gelatin did not support significant growth of any of the isolates. These isolates extend the diversity of known HAP rumen bacteria and indicate the presence of significant HAP bacterial populations in pasture-grazed New Zealand ruminants.
Keyword Quantitative digestion
Microorganisms invitro
Proteolytic Activity
Rumen Bacteria
Fresh herbage
Ribosomal-rna
Protein
Identification
Inhibitors
Tetronasin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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