A modified parallel artificial membrane permeability assay for evaluating the bioconcentration of highly hydrophobic chemicals in fish

Kwon, J. H. and Escher, B. I. (2008) A modified parallel artificial membrane permeability assay for evaluating the bioconcentration of highly hydrophobic chemicals in fish. Environmental Science and Technology, 42 5: 1787-1793.


Author Kwon, J. H.
Escher, B. I.
Title A modified parallel artificial membrane permeability assay for evaluating the bioconcentration of highly hydrophobic chemicals in fish
Journal name Environmental Science and Technology   Check publisher's open access policy
ISSN 0013-936X
Publication date 2008-03
Sub-type Article (original research)
DOI 10.1021/es072088n
Volume 42
Issue 5
Start page 1787
End page 1793
Total pages 7
Place of publication Washington, D.C.
Publisher American Chemical Society
Language eng
Subject 05 Environmental Sciences
0502 Environmental Science and Management
Abstract Low cost in vitro tools are needed at the screening stage of assessment of bioaccumulation potential of new and existing chemicals because the number of chemical substances that needs to be tested highly exceeds the capacity of in vivo bioconcentration tests. Thus, the parallel artificial membrane permeability assay (PAMPA) system was modified to predict passive uptake/elimination rate in fish. To overcome the difficulties associated with low aqueous solubility and high membrane affinity of highly hydrophobic chemicals, we measured the rate of permeation from the donor poly(dimethylsiloxane) (PDMS) disk to the acceptor PDMS disk through aqueous and PDMS membrane boundary layers and term the modified PAMPA system “PDMS-PAMPA”. Twenty chemicals were selected for validation of PDMS-PAMPA. The measured permeability is proportional to the passive elimination rate constant in fish and was used to predict the “minimum” in vivo elimination rate constant. The in vivo data were very close to predicted values except for a few polar chemicals and metabolically active chemicals, such as pyrene and benzo[a]pyrene. Thus, PDMS-PAMPA can be an appropriate in vitro system for nonmetabolizable chemicals. Combination with metabolic clearance rates using a battery of metabolic degradation assays would enhance the applicability for metabolizable chemicals.
Keyword Polycyclic Aromatic-hydrocarbons
Zebrafish brachydanio-rerio
Solid-phase Microextraction
Organic-chemicals
In-vivo
Partitiion-coefficients
Lipophilic compounds
Poecilia-reticulata
Passive absorption
Rainbow-trout
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
National Research Centre for Environmental Toxicology Publications
 
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