Development of an in vitro system for modeling bioaccumulation of neutral, ionizable, and metabolically active organic pollutants in fish

Escher, Beate and Kwon, Jung-Hwan (2007) Development of an in vitro system for modeling bioaccumulation of neutral, ionizable, and metabolically active organic pollutants in fish. ALTEX: Alternatives to Animal Experimentation, 24 Supp.: 81-82.

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Author Escher, Beate
Kwon, Jung-Hwan
Title Development of an in vitro system for modeling bioaccumulation of neutral, ionizable, and metabolically active organic pollutants in fish
Formatted title
Development of an in vitro system for modeling bioaccumulation of neutral, ionizable, and metabolically active organic pollutants in fish
Journal name ALTEX: Alternatives to Animal Experimentation   Check publisher's open access policy
ISSN 1868-596X
1868-8551
0946-7785
Publication date 2007
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 24
Issue Supp.
Start page 81
End page 82
Total pages 2
Editor Franz Gruber
Place of publication Heidelberg, Germany
Publisher Springer Spektrum
Language eng
Subject 05 Environmental Sciences
0502 Environmental Science and Management
Formatted abstract
Background and aim The pending implementation of the new European chemicals legislation (REACH) will produce the requirement of a lot of additional testing, including bioaccumulation assessment in fish and other aquatic species. Bioconcentration testing is highly animal intensive. Thus alternative test methods have to be developed to reduce the number of test animals or to avoid the use of test animals by using alternative in vitro test systems.

Bioaccumulation encompasses bioconcentration, i.e. the passive uptake (in fish via the gills), and biomagnification, i.e., the uptake via ingestion of contaminated food. Bioconcentration integrates the uptake, distribution and elimination of a substance due to water-borne exposure. In fish, bioaccumulation is typically dominated by bioconcentration due to the high surface area of the gill membranes. Metabolism decreases the BCF. Thus assessment models and in vitro methods should account for metabolism. The in vitro assay to be developed should therefore account for metabolic processes in fish.

The main goal of this project is the refinement of a new in vitro method for evaluating bioconcentration kinetics in fish using the parallel artificial membrane permeability assay (PAMPA) to assess membrane permeation and membrane-water partitioning. With this information it should be possible to develop a prediction model to replace animal testing with the OECD 305 fish bioconcentration test.
Keyword Fish
Ecotoxicology
Biochemical / analytical
In silico
Reduction
Replacement
Drug screening
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
National Research Centre for Environmental Toxicology Publications
 
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Created: Tue, 17 Nov 2009, 09:47:07 EST