Prevalence of staphylococcus aureus strains in an australian cohort, 1989-2003: evidence for the low prevalence of the toxic shock toxin and panton-valentine leukocidin genes

Schlebusch, S., Schooneveldt, J.M., Huygens, F. and Nimmo, G.R. (2009) Prevalence of staphylococcus aureus strains in an australian cohort, 1989-2003: evidence for the low prevalence of the toxic shock toxin and panton-valentine leukocidin genes. European Journal of Clinical Microbiology & Infectious Diseases, 28 10: 1183-1189. doi:10.1007/s10096-009-0761-1


Author Schlebusch, S.
Schooneveldt, J.M.
Huygens, F.
Nimmo, G.R.
Title Prevalence of staphylococcus aureus strains in an australian cohort, 1989-2003: evidence for the low prevalence of the toxic shock toxin and panton-valentine leukocidin genes
Journal name European Journal of Clinical Microbiology & Infectious Diseases   Check publisher's open access policy
ISSN 0934-9723
Publication date 2009-10
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s10096-009-0761-1
Volume 28
Issue 10
Start page 1183
End page 1189
Total pages 7
Editor Alex van Belkum
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2010
Language eng
Subject 110309 Infectious Diseases
920109 Infectious Diseases
C1
Abstract The purpose of this paper is to determine the prevalence of the toxic shock toxin gene (tst) and to enumerate the circulating strains of methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in Australian isolates collected over two decades. The aim was to subtype these strains using the binary genes pvl, cna, sdrE, pUB110 and pT181. Isolates were assayed using real-time polymerase chain reaction (PCR) for mecA, nuc, 16 S rRNA, eight single-nucleotide polymorphisms (SNPs) and for five binary genes. Two real-time PCR assays were developed for tst. The 90 MRSA isolates belonged to CC239 (39 in 1989, 38 in 1996 and ten in 2003), CC1 (two in 2003) and CC22 (one in 2003). The majority of the 210 MSSA isolates belonged to CC1 (26), CC5 (24) and CC78 (23). Only 18 isolates were tst-positive and only 15 were pvl-positive. Nine MSSA isolates belonged to five binary types of ST93, including two pvl-positive types. The proportion of tst-positive and pvl-positive isolates was low and no significant increase was demonstrated. Dominant MSSA clonal complexes were similar to those seen elsewhere, with the exception of CC78. CC239 MRSA (AUS-2/3) was the predominant MRSA but decreased significantly in prevalence, while CC22 (EMRSA-15) and CC1 (WA-1) emerged. Genetically diverse ST93 MSSA predated the emergence of ST93-MRSA (the Queensland clone).
Keyword Staphylococcus Aureus
Infectious diesase
Microbiology
Panton-valentine Leukocidin
toxic shock toxin gene
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
2010 Higher Education Research Data Collection
 
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Created: Thu, 12 Nov 2009, 12:04:06 EST by Mr Andrew Martlew on behalf of UQ Centre for Clinical Research