Expression of prohormone convertase 2 and the generation of neuropeptides in the developing nervous system of the gastropod Haliotis

Cummins, SF, York, PS, Hanna, PJ, Degnan, BM and Croll, RP (2009) Expression of prohormone convertase 2 and the generation of neuropeptides in the developing nervous system of the gastropod Haliotis. International Journal of Developmental Biology, 53 7: 1081-1088. doi:10.1387/ijdb.082791sc


Author Cummins, SF
York, PS
Hanna, PJ
Degnan, BM
Croll, RP
Title Expression of prohormone convertase 2 and the generation of neuropeptides in the developing nervous system of the gastropod Haliotis
Formatted title
Expression of prohormone convertase 2 and the generation of neuropeptides in the developing nervous system of the gastropod Haliotis
Journal name International Journal of Developmental Biology   Check publisher's open access policy
ISSN 0214-6282
Publication date 2009
Sub-type Article (original research)
DOI 10.1387/ijdb.082791sc
Volume 53
Issue 7
Start page 1081
End page 1088
Total pages 7
Editor Juan Arechaga
Place of publication Spain
Publisher Universidad del Pais Vasco, Servicio Editorial
Collection year 2010
Language eng
Subject C1
060305 Evolution of Developmental Systems
830103 Aquaculture Molluscs (excl. Oysters)
Formatted abstract
Prohormone convertase 2 (PC2) belongs to a family of enzymes involved in the
proteolytic maturation of neuropeptide precursors into mature peptides that act as neurotransmitters,
neuromodulators or neurohormones. Here we show that a gene encoding a PC2-like
enzyme (HasPC2) is expressed during larval development and in the adult ganglia of the
vetigastropod Haliotis asinina. HasPC2 exhibits high sequence identity to other gastropod PC2s
and thus is likely to function in peptide processing. Analysis of HasPC2 expression indicates that
it is activated early in nervous system development. During trochophore and early veliger larval
stages, HasPC2 is expressed in the vicinity of the forming ganglia of the central nervous system
and parts of the putative peripheral nervous system. Later in larval development, at the time the
veliger becomes competent to interact with the external environment and initiate metamorphosis,
HasPC2 expression largely restricts to cells of the major ganglia and their commissures.
Profiling of veliger larvae by bioinformatic approaches suggests the expression of a variety of
peptides. Direct MALDI-MS-based peptide profiling of juvenile Haliotis cerebral ganglia (brain)
reveals an abundance of neuropeptides, including FMRFamide-related peptides and APGWamide,
compatible with PC2 functioning in neuropeptide processing in these regions. These results are
consistent with PC2 regulating neuropeptide generation in the earliest functioning of the
gastropod nervous system.
Keyword prohormone convertase
abalone
neuropeptide
development
FMRFAMIDE-RELATED PEPTIDES
DEVELOPMENTAL EXPRESSION
SEQUENCE ALIGNMENT
MOLECULAR-CLONING
CONSERVED ROLE
ASININA
GENE
CDNA
PC2
CATECHOLAMINES
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Thu, 12 Nov 2009, 12:03:04 EST by Mr Andrew Martlew on behalf of School of Biological Sciences