Synthesis and Application of FRET Nanoparticles in the Profiling of a Protease

Marcon, L., Spriet, C., Meehan, T.D., Battersby, B.J., Lawrie, G.A., Heliot, L. and Trau, M. (2009) Synthesis and Application of FRET Nanoparticles in the Profiling of a Protease. Small, 5 18: 2053-2056. doi:10.1002/smll.200801887


Author Marcon, L.
Spriet, C.
Meehan, T.D.
Battersby, B.J.
Lawrie, G.A.
Heliot, L.
Trau, M.
Title Synthesis and Application of FRET Nanoparticles in the Profiling of a Protease
Journal name Small   Check publisher's open access policy
ISSN 1613-6810
Publication date 2009-09-18
Year available 2009
Sub-type Article (original research)
DOI 10.1002/smll.200801887
Volume 5
Issue 18
Start page 2053
End page 2056
Total pages 4
Editor Jose Oliveira
Lisa Wylie
Place of publication Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Collection year 2010
Language eng
Subject C1
Formatted abstract
Fluorescent silica nanoparticles incorporating unique ratios of energy-transfer dyes are synthesized and applied as colloidal barcodes to encode a microsphere-bound combinatorial peptide library. The affinity of the West Nile virus protease is profiled using this library with cleavage of the peptide detected by flow cytometry. The cleaved peptide substrates are sorted and then identified through decoding by confocal microscopy combined with spectral unmixing (see image). ©2009 Wiley-VCH GmbH & Co. KGaA, Weinheim
Keyword barcoding
confocal microscopy
fluorescence
nanoparticles
protease
WEST-NILE-VIRUS
SINGLE-WAVELENGTH EXCITATION
COMBINATORIAL CHEMISTRY
SILICA NANOPARTICLES
DRUG DISCOVERY
LIBRARIES
MICROSPHERES
FLUOROPHORES
COLLOIDS
DENGUE
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 12 Nov 2009, 21:59:54 EST by Mr Andrew Martlew on behalf of Aust Institute for Bioengineering & Nanotechnology