A lipase-like gene from Heliothis virescens ascovirus (HvAV-3e) is essential for virus replication and cell cleavage

Smede, M, Hussain, M and Asgari, S (2009) A lipase-like gene from Heliothis virescens ascovirus (HvAV-3e) is essential for virus replication and cell cleavage. Virus Genes, 39 3: 409-417. doi:10.1007/s11262-009-0407-5


Author Smede, M
Hussain, M
Asgari, S
Title A lipase-like gene from Heliothis virescens ascovirus (HvAV-3e) is essential for virus replication and cell cleavage
Formatted title A lipase-like gene from Heliothis virescens ascovirus (HvAV-3e) is essential for virus replication and cell cleavage
Journal name Virus Genes   Check publisher's open access policy
ISSN 0920-8569
Publication date 2009-11
Sub-type Article (original research)
DOI 10.1007/s11262-009-0407-5
Volume 39
Issue 3
Start page 409
End page 417
Total pages 9
Editor Yechiel Becker
Place of publication United States
Publisher Springer New York LLC
Collection year 2010
Language eng
Subject 970106 Expanding Knowledge in the Biological Sciences
060506 Virology
C1
Abstract uq
Formatted abstract A unique feature of ascovirus infection is
cleavage of host cells into virus containing vesicles. It has
been suggested that the virus induces apoptosis, either by
expression of a caspase or other means, which is then
diverted toward vesicle formation. There is little known
about the mechanism of vesicle formation. Recent genome
sequences of three ascoviruses indicated the presence of
several putative open reading frames coding for proteins
that could be involved in lipid metabolism. These proteins
may play a role in rearrangement of membranes in infected
host cells leading to formation of vesicles. Here, we analyzed
a lipase-like gene (ORF19) from Heliothis virescens
ascovirus (HvAV-3e) expressed from 8 h after infection
and essential for virus replication and cell cleavage. In
addition, ORF19 knock down by RNA interference inhibited
virus replication indicating that the gene is indispensable
for HvAV-3e replication. However, under
enzymatic assays tested, we did not detect any lipase or
esterase activity from ORF19.
Keyword Ascovirus
Insect virus
Heliothis virescens ascovirus
Lipase
Esterase
DNA INSECT VIRUS
SPODOPTERA-FRUGIPERDA
ENVELOPE PROTEIN
VACCINIA VIRUS
SEQUENCE
GENOME
HISTOPATHOLOGY
ORGANIZATION
INFECTIVITY
PREDICTION
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Thu, 12 Nov 2009, 11:55:46 EST by Mr Andrew Martlew on behalf of School of Biological Sciences