The role of actin filaments in ascovirus replication and pathology

Hussain, M, Garrad, S and Asgari, S (2009) The role of actin filaments in ascovirus replication and pathology. Archives of Virology, 154 11: 1737-1743. doi:10.1007/s00705-009-0512-8


Author Hussain, M
Garrad, S
Asgari, S
Title The role of actin filaments in ascovirus replication and pathology
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
Publication date 2009-11
Sub-type Article (original research)
DOI 10.1007/s00705-009-0512-8
Volume 154
Issue 11
Start page 1737
End page 1743
Total pages 7
Editor M H V Van Regenmortel
Place of publication Austria
Publisher Springer Wien
Collection year 2010
Language eng
Subject 970106 Expanding Knowledge in the Biological Sciences
060506 Virology
C1
Formatted abstract
Ascoviruses (AVs) are insect viruses transmitted
by parasitoid wasps. The unique pathology in host cells
upon AV infection includes enlargement, blebbing and
cleavage of host cells into virus-containing vesicles that are
important in dissemination of the virus. The mechanism of
pathogenesis and vesicle formation is largely unknown.
Here, we explored involvement of actin filaments in virus
entry, replication and pathology. The results suggested that
entry of Heliothis virescens ascovirus-3e (HvAV-3e) leads
to rearrangement of the actin cytoskeleton. After HvAV-3e
infection, actin filaments were found in foci rather than in a
homogenous distribution within the cytoplasm. Actin filaments
were also found concentrating around blebs and
vesiculation areas of the cell cortex following infection.
Destabilization of filamentous actin by cytochalasin D did
not inhibit entry or replication of the virus but affected
vesiculation and pathology associated with HvAV-3e
infection. These observations suggested that actin may not
be required for virus entry and replication but essential for
virus pathology, mainly vesicle formation.
Keyword HELIOTHIS-VIRESCENS ASCOVIRUS
DNA INSECT VIRUS
CELL-LINES
APOPTOSIS
SEQUENCE
TRANSMISSION
ORGANIZATION
CYTOSKELETON
INFECTIVITY
GENOME
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Thu, 12 Nov 2009, 11:55:20 EST by Mr Andrew Martlew on behalf of School of Biological Sciences