Effect of Motif-Programmed Artificial Proteins on the Calcium Uptake in a Synthetic Hydrogel

Chirila, T.V., Minamisawa, T., Keen, I. and Shiba, K. (2009) Effect of Motif-Programmed Artificial Proteins on the Calcium Uptake in a Synthetic Hydrogel. Macromolecular Bioscience, 9 10: 959-967. doi:10.1002/mabi.200900096


Author Chirila, T.V.
Minamisawa, T.
Keen, I.
Shiba, K.
Title Effect of Motif-Programmed Artificial Proteins on the Calcium Uptake in a Synthetic Hydrogel
Journal name Macromolecular Bioscience   Check publisher's open access policy
ISSN 1616-5195
Publication date 2009-04-28
Year available 2009
Sub-type Article (original research)
DOI 10.1002/mabi.200900096
Volume 9
Issue 10
Start page 959
End page 967
Total pages 9
Editor Joern Ritterbusch
Place of publication Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Collection year 2010
Language eng
Subject C1
970103 Expanding Knowledge in the Chemical Sciences
920203 Diagnostic Methods
100799 Nanotechnology not elsewhere classified
Abstract Motif-programmed artificial proteins with mineralization-related activity were covalently immobilized onto the surface of a hydrogel, poly(2-hydroxyethyl methacrylate) (PHEMA). We investigated the influence of assaying conditions upon the ability of three selected proteins (PS64, PS382 and PS458) to modulate calcification in vitro. A long-term assay measuring the real amount of calcium phosphate phase in the protein-modified PHEMA showed that all proteins enhanced the uptake of calcium by the hydrogel. For PS382 and PS458, this is a behaviour opposite to that displayed when the same proteins were tested in a free state by a rapid solution assay. Such difference may be attributed to a restricted mobility of the proteins due to immobilization.
Keyword biomimetic
calcification
ESCA/XPS
immobilization
PHEMA hydrogel
proteins
X-RAY PHOTOELECTRON
DENTIN MATRIX PROTEIN-1
MUSSEL PINNA-NOBILIS
SULFONYL CHLORIDES
ACRYLIC HYDROGELS
CODING SEQUENCES
EPITHELIAL-CELLS
PINCTADA-FUCATA
PEPTIDE MOTIFS
IN-VITRO
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Thu, 12 Nov 2009, 21:51:25 EST by Mr Andrew Martlew on behalf of Medicine - Princess Alexandra Hospital