Acibenzolar-S-methyl-induced alteration of defence gene expression and enzyme activity in cotton infected with Fusarium oxysporum f. sp. vasinfectum

Whan, Jennifer A., Dann, Elizabeth K., Smith, Linda J. and Aitken, Elizabeth A. B. (2008) Acibenzolar-S-methyl-induced alteration of defence gene expression and enzyme activity in cotton infected with Fusarium oxysporum f. sp. vasinfectum. Physiological and Molecular Plant Pathology, 73 6: 175-182.


Author Whan, Jennifer A.
Dann, Elizabeth K.
Smith, Linda J.
Aitken, Elizabeth A. B.
Title Acibenzolar-S-methyl-induced alteration of defence gene expression and enzyme activity in cotton infected with Fusarium oxysporum f. sp. vasinfectum
Formatted title Acibenzolar-S-methyl-induced alteration of defence gene expression and enzyme activity in cotton infected with Fusarium oxysporum f. sp. vasinfectum
Journal name Physiological and Molecular Plant Pathology   Check publisher's open access policy
ISSN 0885-5765
1096-1178
Publication date 2008-11
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.pmpp.2009.06.003
Volume 73
Issue 6
Start page 175
End page 182
Total pages 8
Place of publication London, United Kingdom
Publisher Academic Press
Collection year 2010
Language eng
Subject C1
060704 Plant Pathology
820301 Cotton
Formatted abstract The effect of acibenzolar-S-methyl (ASM) applied in the form of Bion® as a seed soak or a foliar spray, on transcript accumulation of six defence related genes, and activities of four defence related enzymes, was assessed after cotton was inoculated with Fusarium oxysporum f. sp. vasinfectum (Fov). Significant upregulation of chitinase class I, peroxidase, and β-1, 3-glucanase transcripts occurred in the Bion® seed soak treatment with Fov inoculation compared to all other treatments. Bion® alone did not significantly increase transcripts compared with water control treatments in the majority of cases. Phenylalanine ammonia lyase transcript in Bion® treated plants following fungal inoculation was greater than in the respective Bion® alone treatments, and osmotin-like protein transcript was significantly greater in inoculated plants sprayed with Bion® than in all other treatments. Transcripts of 4-coumarate-CoA ligase-like protein were generally lower than for all other genes, and significant upregulation compared with water control occurred in extracts from Fov inoculated plants receiving no Bion® treatment, and Fov inoculated plants cultivated from Bion® treated seed. The greatest increases in chitinase, β-1, 3-glucanase and peroxidase activity occurred in root and shoot tissue from Bion® seed treated material inoculated with Fov. Bion® treatment alone did not consistently enhance enzyme activities above those measured in water treated controls. Polyphenol oxidase activities were enhanced by various Bion® or Fov treatments at 3 days after inoculation only.
Keyword Fusarium oxysporum f. sp. vasinfectum
Bion®
Acibenzolar-S-methyl (ASM)
Cotton
Induced defence
Real-time RT-PCR
Transcript accumulation
Chitinase
Phenylalanine ammonia lyase
β-1
3-Glucanase
Polyphenol oxidase
Peroxidase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 7 July 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 5 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 191 Abstract Views  -  Detailed Statistics
Created: Wed, 11 Nov 2009, 15:11:44 EST by Hayley Ware on behalf of School of Biological Sciences