Determination of para-aminohippuric acid (PAH) in human plasma and urine by liquid chromatography-tandem mass spectrometry

Han, Phey Yen, Shaw, P. Nicholas and Kirkpatrick, Carl M. J. (2009) Determination of para-aminohippuric acid (PAH) in human plasma and urine by liquid chromatography-tandem mass spectrometry. Journal of Chromatography B, 877 27: 3215-3220. doi:10.1016/j.jchromb.2009.08.037


Author Han, Phey Yen
Shaw, P. Nicholas
Kirkpatrick, Carl M. J.
Title Determination of para-aminohippuric acid (PAH) in human plasma and urine by liquid chromatography-tandem mass spectrometry
Formatted title
Determination of para-aminohippuric acid (PAH) in human plasma and urine by liquid chromatography-tandem mass spectrometry
Journal name Journal of Chromatography B   Check publisher's open access policy
ISSN 1570-0232
1873-376X
Publication date 2009-10-01
Sub-type Article (original research)
DOI 10.1016/j.jchromb.2009.08.037
Volume 877
Issue 27
Start page 3215
End page 3220
Total pages 6
Editor G. Hopfgartner
H. T. Karnes
R. P. H. Bischoff
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2010
Language eng
Subject C1
030101 Analytical Spectrometry
920203 Diagnostic Methods
Formatted abstract
In this manuscript, we present a simple and reliable method for the quantitation of para-aminohippuric acid (PAH; 2-(4-aminobenzamido)acetic acid) in human plasma and urine using liquid chromatography coupled to electrospray ionisation low-energy collision-induced dissociation tandem mass spectrometry (HPLC–ESI-CID-MS/MS) analysis (negative ion mode) via multiple reaction monitoring (MRM). Sample preparation involved protein precipitation of plasma samples with acetonitrile and subsequent dilution of urine samples with the mobile phase. The internal standard (IS) adopted was para-aminosalicylic acid (PAS; 4-amino-2-hydroxybenzoic acid). Chromatographic separation was achieved on a Cosmosil HILIC column using an isocratic mobile phase consisting of ammonium acetate buffer (20 mM) and acetonitrile (45:55, v/v) at a flow rate of 200 μl/min. The transactions monitored were m/z 192.9 → 149.1 for PAH and m/z 152.1 → 108.1 for IS. Linear calibration curves were generated over the PAH concentration range of 0.2–100 mg/L in human plasma and urine. The method was validated for selectivity, accuracy, precision, recovery and stability according to USFDA criteria, and has been successfully applied to a pharmacokinetic study in healthy volunteers administered an intravenous dose of 440 mg PAH.
Keyword para-Aminohippuric acid
High-performance liquid chromatography
Mass spectrometry
Human plasma
Human urine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
ERA 2012 Admin Only
School of Pharmacy Publications
 
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Created: Tue, 10 Nov 2009, 14:49:46 EST by Charna Kovacevic on behalf of School of Pharmacy