Cholesterol induces mitochondrial dysfunction and apoptosis in mouse pancreatic beta-cell line MIN6 cells

Zhao, Yu-Feng, Wang, Li, Lee, Soohyun, Sun, Qiang, Tuo, Ya, Wang, Yuemin, Pei, Jianming and Chen, Chen (2010) Cholesterol induces mitochondrial dysfunction and apoptosis in mouse pancreatic beta-cell line MIN6 cells. Endocrine, 37 1: 76-82. doi:10.1007/s12020-009-9275-y


Author Zhao, Yu-Feng
Wang, Li
Lee, Soohyun
Sun, Qiang
Tuo, Ya
Wang, Yuemin
Pei, Jianming
Chen, Chen
Title Cholesterol induces mitochondrial dysfunction and apoptosis in mouse pancreatic beta-cell line MIN6 cells
Journal name Endocrine   Check publisher's open access policy
ISSN 1355-008X
1559-0100
Publication date 2010-02
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s12020-009-9275-y
Volume 37
Issue 1
Start page 76
End page 82
Total pages 6
Editor P. Michael Conn
Place of publication United States
Publisher Humana Press, Inc.
Collection year 2010
Language eng
Subject C1
920106 Endocrine Organs and Diseases (excl. Diabetes)
110306 Endocrinology
110201 Cardiology (incl. Cardiovascular Diseases)
Abstract Reduction of pancreatic beta-cell mass is a key element leading to type 2 diabetes. Obesity and overweight with high levels of lipids including cholesterol are tightly linked to type 2 diabetes. The direct impact of cholesterol on pancreatic beta-cells, however, has not been extensively studied. In this study, MIN6 mouse beta-cell line was used to test the effect of cholesterol on pancreatic beta-cell apoptosis over different doses and durations. It was found that cholesterol dose- and time-dependently induced cell death of MIN6 cells above 160 muM after 6 h treatment in vitro. Annexin-V staining revealed that cholesterol treatment significantly induced apoptosis in MIN6 cells. Cholesterol treatment resulted in the loss of the ability to retain Rhodamine 123, indicating mitochondrial damage in MIN6 cells. Cholesterol-induced cell apoptosis and mitochondrial damage were blocked by low-temperature condition. In addition, glutathione also protected MIN6 cells from cholesterol-induced cell death. It is concluded that high level of cholesterol induces cell apoptosis in MIN6 cells, which is in part due to mitochondrial dysfunction. We suggest that excessive uptake of cholesterol in beta-cells may contribute to beta-cell apoptosis and dysfunction and the deterioration of type 2 diabetes.
Keyword Cholesterol
Mitochondria
Apoptosis
β-cells
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Mon, 09 Nov 2009, 13:53:30 EST by Cameron Harris on behalf of Faculty of Science