Differential plasma protein binding to metal oxide nanoparticles

Deng, Zhou J., Mortimer, Gysell, Schiller, Tara, Musumeci, Anthony, Martin, Darren and Minchin, Rodney F. (2009) Differential plasma protein binding to metal oxide nanoparticles. Nanotechnology, 20 45: 455101.1-455101.9. doi:10.1088/0957-4484/20/45/455101


Author Deng, Zhou J.
Mortimer, Gysell
Schiller, Tara
Musumeci, Anthony
Martin, Darren
Minchin, Rodney F.
Title Differential plasma protein binding to metal oxide nanoparticles
Journal name Nanotechnology   Check publisher's open access policy
ISSN 1361-6528
0957-4484
Publication date 2009-10-13
Year available 2009
Sub-type Article (original research)
DOI 10.1088/0957-4484/20/45/455101
Volume 20
Issue 45
Start page 455101.1
End page 455101.9
Total pages 9
Editor Mark Reed
Place of publication Bristol, United Kingdom
Publisher Institute of Physics Publishing
Collection year 2010
Language eng
Subject C1
9201 Clinical Health (Organs, Diseases and Abnormal Conditions)
111201 Cancer Cell Biology
111501 Basic Pharmacology
111505 Pharmacogenomics
Abstract Nanoparticles rapidly interact with the proteins present in biological fluids, such as blood. The proteins that are adsorbed onto the surface potentially dictate the biokinetics of the nanomaterials and their fate in vivo. Using nanoparticles with different sizes and surface characteristics, studies have reported the effects of physicochemical properties on the composition of adsorbed plasma proteins. However, to date, few studies have been conducted focusing on the nanoparticles that are commonly exposed to the general public, such as the metal oxides. Using previously established ultracentrifugation approaches, two-dimensional gel electrophoresis and mass spectrometry, the current study investigated the binding of human plasma proteins to commercially available titanium dioxide, silicon dioxide and zinc oxide nanoparticles. We found that, despite these particles having similar surface charges in buffer, they bound different plasma proteins. For TiO2, the shape of the nanoparticles was also an important determinant of protein binding. Agglomeration in water was observed for all of the nanoparticles and both TiO2 and ZnO further agglomerated in biological media. This led to an increase in the amount and number of different proteins bound to these nanoparticles. Proteins with important biological functions were identified, including immunoglobulins, lipoproteins, acute-phase proteins and proteins involved in complement pathways and coagulation. These results provide important insights into which human plasma proteins bind to particular metal oxide nanoparticles. Because protein absorption to nanoparticles may determine their interaction with cells and tissues in vivo, understanding how and why plasma proteins are adsorbed to these particles may be important for understanding their biological responses.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article # 455101

 
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Created: Thu, 22 Oct 2009, 13:58:46 EST by Cameron Harris on behalf of School of Biomedical Sciences