Gene expression profiling during Forskolin induced differentiation of BeWo cells by differential display RT-PCR

Neelima, P. S. and Rao, A. J. (2008) Gene expression profiling during Forskolin induced differentiation of BeWo cells by differential display RT-PCR. Molecular and Cellular Endocrinology, 281 1-2: 37-46. doi:10.1016/j.mce.2007.10.002


Author Neelima, P. S.
Rao, A. J.
Title Gene expression profiling during Forskolin induced differentiation of BeWo cells by differential display RT-PCR
Journal name Molecular and Cellular Endocrinology   Check publisher's open access policy
ISSN 0303-7207
1872-8057
Publication date 2008-01-16
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.mce.2007.10.002
Volume 281
Issue 1-2
Start page 37
End page 46
Total pages 10
Place of publication Shannon, Co. Clare Ireland
Publisher Elsevier Ireland
Language eng
Subject 060103 Cell Development, Proliferation and Death
060405 Gene Expression (incl. Microarray and other genome-wide approaches)
Abstract The differentiation of cytotrophoblasts into syncytiotrophoblasts in the placenta has been employed as a model to investigate stage specific expression as well as regulation of genes during this process. While the cytotrophoblasts are highly invasive and proliferative with relatively less capacity to synthesize pregnancy related proteins, the multinucleated syncytiotrophoblasts are non-proliferative and non-invasive. However, syncytiotrophoblasts are the site of synthesis of a variety of protein, peptide and steroid hormones as well as several growth factors. Both the freshly isolated cytotrophoblasts from human placenta as well as the BeWo cell, a choriocarcinoma cell line model which retain several characteristic of cytotrophoblasts has been employed by us to study regulation of differentiation. In the present study, we have employed the differential display RT-PCR analysis (DD-RT-PCR) to evaluate gene expression changes during Forskolin induced in vitro differentiation of BeWo cells. We have identified several genes which are differentially expressed during differentiation and the differential expression of 10 transcripts was confirmed by Northern blot analysis. Based on the identity of the transcripts an attempt has been made to relate the known function of the gene products, to changes observed during differentiation. Of the several transcripts, one of the transcripts, namely Secretory Leukocyte Protease Inhibitor (SLPI) which is known to have multiple functions was found to increase 15-fold in the syntiotrophoblast.
Keyword BeWo cells
DD-RT-PCR
Cell differentiation
SLPI
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Mon, 12 Oct 2009, 12:41:40 EST by Ms Lynette Adams on behalf of Institute for Molecular Bioscience