Comparative osteogenic transcription profiling of various fetal and adult mesenchymal stem cell sources

Guillot, Pascale V., De Bari, Cosimo, Dell'Accio, Francesco, Kurata, Hitoshi, Polak, Julia and Fisk, Nicholas M. (2008) Comparative osteogenic transcription profiling of various fetal and adult mesenchymal stem cell sources. Differentiation, 76 9: 946-957. doi:10.1111/j.1432-0436.2008.00279.x

Author Guillot, Pascale V.
De Bari, Cosimo
Dell'Accio, Francesco
Kurata, Hitoshi
Polak, Julia
Fisk, Nicholas M.
Title Comparative osteogenic transcription profiling of various fetal and adult mesenchymal stem cell sources
Journal name Differentiation   Check publisher's open access policy
ISSN 0301-4681
Publication date 2008-11
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1432-0436.2008.00279.x
Volume 76
Issue 9
Start page 946
End page 957
Total pages 12
Editor Gerald R. Cunha
Helen M. Blau
Eero Lehtonen
Place of publication Berlin, Germany
Publisher Elsevier
Collection year 2010
Language eng
Subject 0601 Biochemistry and Cell Biology
Formatted abstract
Human mesenchymal stem cells (MSC) from adult and fetal tissues are promising candidates for cell therapy but there is a need to identify the optimal source for bone regeneration. We have previously characterized MSC populations in first trimester fetal blood, liver, and bone marrow and we now evaluate their osteogenic differentiation potential in comparison to adult bone marrow MSC. Using quantitative real-time RT-PCR, we demonstrated that 16 osteogenic-specific genes (OC, ON, BSP, OP, Col1, PCE, Met2A, OPG, PHOS1, SORT, ALP, BMP2, CBFA1, OSX, NOG, IGFII) were expressed in both fetal and adult MSC under basal conditions and were up-regulated under osteogenic conditions both in vivo and during an in vitro 21-day time-course. However, under basal conditions, fetal MSC had higher levels of osteogenic gene expression than adult MSC. Upon osteogenic differentiation, fetal MSC produced more calcium in vitro and reached higher levels of osteogenic gene up-regulation in vivo and in vitro. Second, we observed a hierarchy within fetal samples, with fetal bone marrow MSC having greater osteogenic potential than fetal blood MSC, which in turn had greater osteogenic potential than fetal liver MSC. Finally, we found that the level of gene expression under basal conditions was positively correlated with both calcium secretion and gene expression after 21 days in osteogenic conditions. Our findings suggest that stem cell therapy for bone dysplasias such as osteogenesis imperfecta may benefit from preferentially using first trimester fetal blood or bone marrow MSC over fetal liver or adult bone marrow MSC.
© 2008 International Society for Differentiation
Keyword Fetal stem cells
Osteogenic-specific genes
Human mesenchymal stem cells
Osteogenic differentiation
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Centre for Clinical Research Publications
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