Assessing indoor air exposures using passive sampling with bioanalytical methods for estrogenicity and aryl hydrocarbon receptor activity

Kennedy, K., Macova, Mirsolva, Leusch, Frederick, Bartkow, Michael E., Hawker, Darryl W., Zhao, Bin, Denison, Michael S. and Mueller, Jochen F. (2009) Assessing indoor air exposures using passive sampling with bioanalytical methods for estrogenicity and aryl hydrocarbon receptor activity. Analytical and Bioanalytical Chemistry, 394 5: 1413-1421.


Author Kennedy, K.
Macova, Mirsolva
Leusch, Frederick
Bartkow, Michael E.
Hawker, Darryl W.
Zhao, Bin
Denison, Michael S.
Mueller, Jochen F.
Title Assessing indoor air exposures using passive sampling with bioanalytical methods for estrogenicity and aryl hydrocarbon receptor activity
Journal name Analytical and Bioanalytical Chemistry  (ERA 2012 Listed)    (ERA 2010 Rank B)   Check publisher's open access policy
Publication date 2009-05
Sub-type Article
Year available 2009
DOI 10.1007/s00216-009-2825-6
Volume number 394
Issue number 5
ISSN 1618-2642
1618-2650
Start page 1413
End page 1421
Total pages 9
Place of publication Berlin / Heidelberg, Germany
Publisher Springer-Verlag
Collection year 2010
Language eng
Subject 050206 Environmental Monitoring
111506 Toxicology (incl.Clinical Toxicology)
030104 Immunological and Bioassay Methods
039901 Environmental Chemistry (incl. Atmospheric Chemistry)
C1
Abstract Passive air sampling was undertaken using polyurethane foam passive air samplers at three types of locations, including indoors (six offices) at buildings in the central business district (CBD) and at a private suburban home (indoor and outdoor) located 9 km from the CBD in Brisbane, Queensland, Australia. Estrogenic (E-SCREEN—MCF7-BOS) and aryl hydrocarbon receptor (AhR) (CAFLUX—H4G1.1c2) activity were assessed for samples collected from each of these locations. The samples were tested either as crude extracts (“untreated”) or were subjected to H2SO4 silica gel (“treated”) for each location in order to determine whether chemicals, which are not resistant to this treatment like polycyclic aromatic hydrocarbons, potentially account for the observed activity. In most cases, H2SO4 treatment resulted in a statistically significant reduction of potency for both endpoints, suggesting that chemicals less resistant to treatment may be responsible for much of the detected biological activity in these locations. Estrogenic potency measurements (<0.22–185 pg m−3) were highest in the indoor offices, followed by the indoor suburban home and finally the outdoor suburban home (which was not estrogenic). Total AhR activity for crude extracts (1.3–10 pg m−3) however was highest for the outdoor suburban home site. Levels of polycyclic aromatic hydrocarbons were monitored indoors and outdoors at the suburban home. At that location, polycyclic aromatic hydrocarbon air concentrations were on average approximately two times higher outdoor than indoor, while AhR potency was five times higher outdoor than indoor. No significant correlation was found between the estrogenic and AhR activity (P = 0.88) for the sites in this study.
Keyword Aryl hydrocarbon receptor activity
Polycyclic Aromatic-hydrocarbons
Bioanalytical methods
Indoor air
Passive air sampling
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Mon, 28 Sep 2009, 21:58:49 EST by Mrs Karen Kennedy on behalf of National Res Centre For Environmental Toxicology