Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted

Forrester, Audrey, Farrell, Helen, Wilkinson, Gavin, Kaye, Jane, Davis-Poynter, Nicholas and Minson, Tony (1992) Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted. Journal of Virology, 66 1: 341-348.

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Author Forrester, Audrey
Farrell, Helen
Wilkinson, Gavin
Kaye, Jane
Davis-Poynter, Nicholas
Minson, Tony
Title Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 1992-01-01
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 66
Issue 1
Start page 341
End page 348
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract A mutant of herpes simplex virus type 1 (HSV-1) in which glycoprotein H (gH) coding sequences were deleted and replaced by the Escherichia coli lacZ gene under the control of the human cytomegalovirus IE-1 gene promoter was constructed. The mutant was propagated in Vero cells which contained multiple copies of the HSV-1 gH gene under the control of the HSV-1 gD promoter and which therefore provide gH in trans following HSV-1 infection. Phenotypically gH-negative virions were obtained by a single growth cycle in Vero cells. These virions were noninfectious, as judged by plaque assay and by expression of I-galactosidase following high-multiplicity infection, but partial recovery of infectivity was achieved by using the fusogenic agent polyethylene glycol. Adsorption of gH-negative virions to cells blocked the adsorption of superinfecting wild-type virus, a result in contrast to that obtained with gD-negative virions (D. C. Johnson and M. W. Ligas, J. Virol. 62:4605-4612, 1988). The simplest conclusion is that gH is required for membrane fusion but not for receptor binding, a conclusion consistent with the conservation of gH in all herpesviruses.
Keyword Herpes Simplex
Virus
Mutation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
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Created: Sat, 12 Sep 2009, 00:34:24 EST by Therese Egan on behalf of Clinical Medical Virology Centre