A cell migration device that maintains a defined surface with no cellular damage during wound edge generation

Doran, M.R., Mills, R.J., Parker, A.J., Landman, K.A. and Cooper-White, J.J. (2009) A cell migration device that maintains a defined surface with no cellular damage during wound edge generation. Lab on a Chip, 9 16: 2364-2369.


Author Doran, M.R.
Mills, R.J.
Parker, A.J.
Landman, K.A.
Cooper-White, J.J.
Title A cell migration device that maintains a defined surface with no cellular damage during wound edge generation
Journal name Lab on a Chip   Check publisher's open access policy
ISSN 1473-0197
Publication date 2009
Year available 2009
Sub-type Article (original research)
DOI 10.1039/b900791a
Volume 9
Issue 16
Start page 2364
End page 2369
Total pages 6
Editor Harp Minhas
Place of publication United Kingdom
Publisher Royal Society of Chemistry
Collection year 2010
Language eng
Subject C1
Abstract Studying the rate of cell migration provides insight into fundamental cell biology as well as a tool to assess the functionality of synthetic surfaces and soluble environments used in tissue engineering. The traditional tools used to study cell migration include the fence and wound healing assays. In this paper we describe the development of a microchannel based device for the study of cell migration on defined surfaces. We demonstrate that this device provides a superior tool, relative to the previously mentioned assays, for assessing the propagation rate of cell wave fronts. The significant advantage provided by this technology is the ability to maintain a virgin surface prior to the commencement of the cell migration assay. Here, the device is used to assess rates of mouse fibroblasts (NIH 3T3) and human osteosarcoma (SaOS2) cell migration on surfaces functionalized with various extracellular matrix proteins as a demonstration that confining cell migration within a microchannel produces consistent and robust data. The device design enables rapid and simplistic assessment of multiple repeats on a single chip, where surfaces have not been previously exposed to cells or cellular secretions.
Keyword ASSAY
MODEL
LINES
MONOLAYER
TUMORS
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Fri, 04 Sep 2009, 10:26:27 EST