Surface-bound stem cell factor and the promotion of hematopoietic cell expansion

Doran, M.R., Markway, B.D., Aird, I.A., Rowlands, A.S., George, P.A., Nielsen, L.K. and Cooper-White, J.J. (2009) Surface-bound stem cell factor and the promotion of hematopoietic cell expansion. BIOMATERIALS, 30 25: 4047-4052. doi:10.1016/j.biomaterials.2009.04.043

Author Doran, M.R.
Markway, B.D.
Aird, I.A.
Rowlands, A.S.
George, P.A.
Nielsen, L.K.
Cooper-White, J.J.
Title Surface-bound stem cell factor and the promotion of hematopoietic cell expansion
Journal name BIOMATERIALS   Check publisher's open access policy
ISSN 0142-9612
Publication date 2009-09
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2009.04.043
Volume 30
Issue 25
Start page 4047
End page 4052
Total pages 6
Editor D.F. Williams
Place of publication Netherlands
Publisher Elsevier BV
Collection year 2010
Language eng
Subject C1
Abstract In vivo, stem cell factor (SCF) exists in both a bound and soluble isoform. It is believed that the bound form is more potent and fundamentally required for the maintenance of hematopoietic stem cells (HSCs). This theory is supported by the observation that steel-Dickie mice lacking the bound isoform of SCF are unable to maintain hematopoiesis and by the fact that bound SCF displayed on the surface of transgenic cells is better able to maintain c-kit activation than soluble SCF. Further work has shown that recombinant SCF molecules, which include a surface-binding domain, are more potent than their soluble equivalent. It is generally assumed that such an elegant approach is necessary to provide the correct molecular orientation and avoid the pitfalls of random cross-linking or the denaturation associated with the adsorption of proteins to surfaces. However, in this work we demonstrate that SCF physisorbed to tissue culture plastic (TCP) is not only bioactive, but more potent than the soluble equivalent. By contrast. cross-linking of SCF via free amines is shown to compromise its bioactivity. These observations demonstrate that simple surface modification solutions cannot be discounted and with the advent of low-cost pharmaceutical grade proteins, they should not be. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
Keyword Growth factors
Surface modification
Bone marrow
Stem cell
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 04 Sep 2009, 10:25:25 EST