Incorporation and translocation of 2-deoxy-2-[F-18]fluoro-D-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system

Hattori, Etsuko, Uchida, Hiroshi, Harada, Norihiro, Ohta, Mari, Tsukada, Hideo, Hara, Yasuhiro and Suzuki, Tetsuya (2008) Incorporation and translocation of 2-deoxy-2-[F-18]fluoro-D-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system. Planta, 227 5: 1181-1186. doi:10.1007/s00425-008-0701-9


Author Hattori, Etsuko
Uchida, Hiroshi
Harada, Norihiro
Ohta, Mari
Tsukada, Hideo
Hara, Yasuhiro
Suzuki, Tetsuya
Title Incorporation and translocation of 2-deoxy-2-[F-18]fluoro-D-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system
Journal name Planta   Check publisher's open access policy
ISSN 0032-0935
1432-2048
Publication date 2008-02-14
Sub-type Article (original research)
DOI 10.1007/s00425-008-0701-9
Volume 227
Issue 5
Start page 1181
End page 1186
Total pages 6
Editor A. Melis
G. Weiglein
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Subject 060199 Biochemistry and Cell Biology not elsewhere classified
Abstract [18F]FDG (2-deoxy-2-[18F]Xuoro-D-glucose) was fed to a sorghum plant [Sorghum bicolor (L.) Moench] from the tip of a leaf and its movement was monitored using a planar positron imaging system (PPIS). [18F]FDG was uptaken from the leaf tip and it was translocated to the basal part of the shoots from where it moved to the roots, the tillers and the sheaths. Autoradiographic analysis of the distribution of 18F, [18F]FDG and/or its metabolites showed translocation to the roots, tillers, and to the leaves that were younger than the supplied leaf. Strong labelling was observed in the basal part of the shoots, in the sheaths, the youngest leaf and the root tips. Our results indicate that [18F]FDG and/or its metabolites were absorbed from the leaf and translocated to the sites where nutrients are required. This strongly suggests that [18F]FDG can be utilised as a tracer to study photoassimilate translocation in the living plant. This is the Wrst report on the use of [18F]FDG, which is routinely used as a probe for clinical diagnosis, to study source to sink translocation of metabolites in whole plants in real time.
Keyword 2-deoxy-2-[F-18]fluoro-D-glucose
Photoassimilate translocation
Planar positron imaging system
Sorghum
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
National Research Centre for Environmental Toxicology Publications
 
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Created: Thu, 03 Sep 2009, 10:19:08 EST by Mr Andrew Martlew on behalf of National Res Centre For Environmental Toxicology