Structure analysis and performance of a microbial community from a contaminated aquifer involved in the complete reductive dechlorination of 1,1,2,2-tetrachloroethane to ethene

Rossetti, Simona, Aulenta, Federico, Majone, Mauro, Crocetti, Gregory and Tandoi, Valter (2008) Structure analysis and performance of a microbial community from a contaminated aquifer involved in the complete reductive dechlorination of 1,1,2,2-tetrachloroethane to ethene. Biotechnology and Bioengineering, 100 2: 240-249. doi:10.1002/bit.21776


Author Rossetti, Simona
Aulenta, Federico
Majone, Mauro
Crocetti, Gregory
Tandoi, Valter
Title Structure analysis and performance of a microbial community from a contaminated aquifer involved in the complete reductive dechlorination of 1,1,2,2-tetrachloroethane to ethene
Journal name Biotechnology and Bioengineering   Check publisher's open access policy
ISSN 0006-3592
1097-0290
Publication date 2008-06-01
Sub-type Article (original research)
DOI 10.1002/bit.21776
Volume 100
Issue 2
Start page 240
End page 249
Total pages 10
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Formatted abstract
An anaerobic microcosm set up with aquifer material from a 1,1,2,2-tetrachloroethane (TeCA) contaminated site and amended with butyrate showed a complete TeCA dechlorination to ethene. A structure analysis of the microbial community was performed by fluorescence in situ hybridization (FISH) with already available and on purpose designed probes from sequences retrieved through 16S rDNA clone library construction. FISH was chosen as identification tool to evaluate in situ whether the retrieved sequences belong to primary bacteria responsible for the biodegradative reactions. FISH probes identified up to 80% of total bacteria and revealed the absence or the marginal presence of known TeCA degraders and the abundance of two well-known H2-utilizing halorespiring bacteria, Sulfurospirillum (32.4 ± 8.6% of total bacteria) and Dehalococcoides spp. (14.8 ± 2.8), thereby providing a strong indication of their involvement in the dechlorination processes. These results were supported by the kinetic and thermodynamic analysis which provided indications that hydrogen was the actual electron donor for TeCA dechlorination. The specific probes, developed in this study, for known dechlorinators (i.e., Geobacter, Dehalobacter, and Sulfurospirillum species) represent a valuable tool for any future in situ bioremediation study as well as a quick and specific investigation tool for tracking their distribution in the field.
Keyword 1,1,2,2-tetrachloroethane
Reductive dechlorination
Fluorescence in situ hybridization
Bioremediation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Advanced Water Management Centre Publications
 
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Created: Thu, 03 Sep 2009, 10:09:09 EST by Mr Andrew Martlew on behalf of Advanced Water Management Centre