Burkholderia cepacia complex epidemiology in persons with cystic fibrosis from Australia and New Zealand

Kidd, Timothy J., Douglas, Joel M., Bergh, Haakon A., Coulter, Chris and Bell, Scott C. (2008) Burkholderia cepacia complex epidemiology in persons with cystic fibrosis from Australia and New Zealand. Research in Microbiology, 159 3: 194-199.


Author Kidd, Timothy J.
Douglas, Joel M.
Bergh, Haakon A.
Coulter, Chris
Bell, Scott C.
Title Burkholderia cepacia complex epidemiology in persons with cystic fibrosis from Australia and New Zealand
Journal name Research in Microbiology   Check publisher's open access policy
ISSN 0923-2508
1769-7123
Publication date 2008-04
Sub-type Article (original research)
DOI 10.1016/j.resmic.2008.01.001
Volume 159
Issue 3
Start page 194
End page 199
Total pages 6
Place of publication Cedex, France
Publisher Elsevier Masson
Language eng
Subject 1108 Medical Microbiology
11 Medical and Health Sciences
Abstract The Burkholderia cepacia complex (Bcc) is a group of significant opportunistic respiratory pathogens which affect people with cystic fibrosis. In this study, we sought to ascertain the epidemiology and geographic species distribution of 116 Bcc isolates collected from people with CF in Australia and New Zealand. We performed a combination of recA-based PCR, amplified rDNA restriction analysis (ARDRA), pulsed-field gel electrophoresis and repetitive extragenic palindromic PCR on each isolate. Each Burkholderia cenocepacia isolate was also screened by PCR for the presence of the B. cepacia epidemic strain marker. One hundred and fourteen isolates were assigned to a species using recA-based PCR and ARDRA. B. cenocepacia, B. multivorans and B. cepacia accounted for 45.7%, 29.3% and 11.2% of the isolates, respectively. Strain analysis of B. cenocepacia revealed that 85.3% of the isolates were unrelated. One related B. cenocepacia strain was identified amongst 15 people. Whilst full details of person-to-person contact was not available, all patients attended CF centres in Queensland (Qld) and New South Wales (NSW). Although person-to-person transmission of B. cenocepacia strains has occurred in Australia, the majority of CF-related Bcc infections in Australia and New Zealand are most likely acquired from the environment. Copyright © 2009 Elsevier Masson SAS. All rights reserved
Keyword Burkholderia cepacia complex
Field gel electrophoresis
Epidemiology
Cystic fibrosis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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