Progesterone and pregnancy status of buffaloes treated with a GNRH agonist

Campanile, G., Vecchio, D., Neglia, G., Di Palo, R., Prandi, A. and D'Occhio, M. J. (2008) Progesterone and pregnancy status of buffaloes treated with a GNRH agonist. Livestock Science, 115 2-3: 242-248. doi:10.1016/j.livsci.2007.08.001


Author Campanile, G.
Vecchio, D.
Neglia, G.
Di Palo, R.
Prandi, A.
D'Occhio, M. J.
Title Progesterone and pregnancy status of buffaloes treated with a GNRH agonist
Journal name Livestock Science   Check publisher's open access policy
ISSN 1871-1413
Publication date 2008-06
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.livsci.2007.08.001
Volume 115
Issue 2-3
Start page 242
End page 248
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
The primary aim of the present study was to establish whether the treatment with a GnRH agonist on Day 5 after AI may result in the formation of an accessory corpus luteum, greater progesterone secretion, and the increased likelihood of pregnancy success in buffaloes. The study was conducted during a period of increasing daylight length when progesterone secretion is suppressed and embryonic mortality is relatively high in buffaloes. In Experiment 1, treatment with a GnRH agonist (buserelin, 12 μg) on Day 5 after AI induced acute increases in circulating concentrations of LH, FSH and oestradiol-17β. Pregnant buffaloes (n = 14) at Day 40 following AI showed an increase (P < 0.01) in milk whey progesterone concentration between Day 5 (310 ± 55 pg/ml) and Day 15 (424 ± 50 pg/ml). The non-pregnant buffaloes (n = 7) showed a decrease (P < 0.01) in progesterone level from Day 5 (410 ± 87 pg/ml) to Day 15 (188 ± 30 pg/ml) following AI. In Experiment 2, the treatment with buserelin (12 μg) on Day 5 after AI induced ovulation in 62% of the buffaloes (31/50) and these buffaloes showed a progressive increase in milk whey progesterone concentration on Day 10, 15 and 20 of pregnancy. Buffaloes that did not ovulate, recorded a relatively constant milk whey progesterone level from Day 10 to Day 20 following AI. Milk whey progesterone concentrations increased after the administration of the GnRH agonist in 97% of the pregnant buffaloes and 68% of the non-pregnant buffaloes. The diameter of the largest follicle in buffaloes that ovulated (ovulated n = 31) (8.9 ± 0.04 mm; range 4.2 – 13.0 mm) did not differ significantly from the diameter of the largest follicle in buffaloes that did not ovulate (not ovulate n = 19) (8.7 ± 0.04 mm; range: 4.0 – 12.0 mm). The latter observation suggested that notional ovulatory size follicles in buffaloes are heterogeneous with respect to stage of follicle maturation and capacity to respond to plasma LH. The present study showed that treatment with a GnRH agonist on Day 5 following AI provides a strategy to increase progesterone secretion and the likelihood of pregnancy in buffaloes mated during periods of increasing daylight length.
Keyword Embryonic mortality
Ovulation
GnRH analogue
Buffalo
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Available online 12 September 2007

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 10:01:13 EST by Mr Andrew Martlew on behalf of School of Agriculture and Food Sciences