A preliminary study of the bioactivity of vegetative proteins extracted from Malaysian Bacillus thuringiensis isolates

Ramasanty, B., Nadarajah, V. D., Soong, Z. K., Lee, H. L. and Mohammed, S. M. (2008) A preliminary study of the bioactivity of vegetative proteins extracted from Malaysian Bacillus thuringiensis isolates. Tropical Biomedicine, 25 1: 64-74.

Author Ramasanty, B.
Nadarajah, V. D.
Soong, Z. K.
Lee, H. L.
Mohammed, S. M.
Title A preliminary study of the bioactivity of vegetative proteins extracted from Malaysian Bacillus thuringiensis isolates
Formatted title
A preliminary study of the bioactivity of vegetative proteins extracted from Malaysian Bacillus thuringiensis isolates
Journal name Tropical Biomedicine   Check publisher's open access policy
ISSN 0127-5720
Publication date 2008-04
Sub-type Article (original research)
Volume 25
Issue 1
Start page 64
End page 74
Total pages 11
Place of publication Kuala Lumpur, Malaysia
Publisher Malaysian Society of Parasitology and Tropical Medicine
Language eng
Formatted abstract
Vegetative proteins from Malaysian strains of Bacillus thuringiensis israelensis strains (Bt 11, Bt 12, Bt 15, Bt 16, Bt 17, Bt 21 and Bt 22) and Bacillus sphaericus H-25 strains (Bs 1 and Bs 2) were screened for haemolytic, cytotoxic and larvicidal activity. SDS-PAGE profiles of the Bacillus thuringiensis strains studied consistently showed major bands of 33-37 kDa and 47 kDa. Bt 16 also showed two bands of 66 kDa and 45 kDa similar to the previously reported binary vegetative protein, Vip1Ac (66 kDa) and Vip 2Ac (45 kDa). Both the Bacillus sphaericus strains showed a 35 kDa band that was similiar to a previously reported vegetative protein, the Mtx2 protein. Bs 2 also contains a 37 kDa band, similar to another vegetative protein, the Mtx 3 protein. With the exception of Bt 17 and Bt 21, vegetative proteins from all Bacillus thuringiensis and Bacillus sphaericus strains were highly haemolytic to human erythrocytes, causing more than 75% haemolysis at the highest concentration of 200 μg/ml. High haemolytic activity was associated with high cytotoxic activity with most of the haemolytic strains being indiscriminately cytotoxic to both CEM-SS (human T lymphoblastoid) and HeLa (human uterus cervical cancer) cell lines. Interestingly, the less haemolytic vegetative proteins from Bt 17 and Bt 21 demonstrated cytotoxic activity comparable to that of the highly haemolytic vegetative proteins. Bt 21 displayed toxicity towards both cell lines while Bt 17 was more toxic towards CEM-SS cells. Bioassay against Aedes aegypti and Culex quinquefasciatus larvae revealed that vegetative proteins from the Bacillus thuringiensis strains had activity against both species of larvae but vegetative proteins from Bacillus sphaericus were weakly larvicidal towards Cx. quinquefasciatus only.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Thu, 03 Sep 2009, 09:59:05 EST by Mr Andrew Martlew on behalf of School of Medicine