Particle-by-particle quantification of protein adsorption onto poly(ethylene glycol) grafted surfaces

Chen, Annie, Kozak, Darby, Battersby, Bronwyn J. and Trau, Matt (2008) Particle-by-particle quantification of protein adsorption onto poly(ethylene glycol) grafted surfaces. Biofouling, 24 4: 267-273. doi:10.1080/08927010802058471


Author Chen, Annie
Kozak, Darby
Battersby, Bronwyn J.
Trau, Matt
Title Particle-by-particle quantification of protein adsorption onto poly(ethylene glycol) grafted surfaces
Journal name Biofouling   Check publisher's open access policy
ISSN 1029-2454
0892-7014
Publication date 2008-06
Sub-type Article (original research)
DOI 10.1080/08927010802058471
Volume 24
Issue 4
Start page 267
End page 273
Total pages 7
Editor Len Evans
Place of publication London, United Kingdom
Publisher Taylor and Francis
Language eng
Subject 030603 Colloid and Surface Chemistry
030304 Physical Chemistry of Materials
Formatted abstract
The use and advantage of flow cytometry as a particle-by-particle, low sampling volume, high-throughput screening technique for quantitatively examining the non-specific adsorption of proteins onto surfaces is presented. The adsorption of three proteins: bovine serum albumin (BSA), immunoglobulin gamma (IgG) and protein G, incubated at room temperature for 2 h onto organosilica particles modified with poly(ethylene glycol) (PEG) of increasing MW (2000, 3400, 6000, 10,000 and 20,000 g mol-1) and grafted amounts (0.14–1.4 mg m-2) was investigated as a model system. Each protein exhibited Langmuir-like, high affinity monolayer limited adsorption on unmodified particles with the proteins reaching surface saturation at 1.8, 4.0 and 2.5 mg m-2 for BSA, IgG and protein G, respectively. Protein adsorption on PEG-modified surfaces was found to decrease with increasing amounts of grafted polymer. PEG grafting amounts 40.6 mg m-2 effectively prevented the adsorption of the larger two proteins (BSA and IgG) while a PEG grafting amount 41.3 mg m-2 was required to prevent the adsorption of the smaller protein G.
Keyword Protein adsorption
Protein antifouling layers
PEG grafting and characterization
Flow cytometry
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Australian Institute for Bioengineering and Nanotechnology Publications
Centre for Nanotechnology and Biomaterials Publications
 
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Created: Thu, 03 Sep 2009, 09:54:18 EST by Mr Andrew Martlew on behalf of Aust Institute for Bioengineering & Nanotechnology