Cryopreservation of Centaurea ultreiae (Compositae) a critically endangered species from Galicia (Spain)

Mallon, Ruben, Bunn, Eric, Turner, Shane Robert and Gonzalez, Maria Luz (2008) Cryopreservation of Centaurea ultreiae (Compositae) a critically endangered species from Galicia (Spain). Cryo-Letters, 29 5: 363-370.

Author Mallon, Ruben
Bunn, Eric
Turner, Shane Robert
Gonzalez, Maria Luz
Title Cryopreservation of Centaurea ultreiae (Compositae) a critically endangered species from Galicia (Spain)
Formatted title
Cryopreservation of Centaurea ultreiae (Compositae) a critically endangered species from Galicia (Spain)
Journal name Cryo-Letters   Check publisher's open access policy
ISSN 0143-2044
1742-0644
Publication date 2008-09
Sub-type Article (original research)
Volume 29
Issue 5
Start page 363
End page 370
Total pages 8
Place of publication Great Marlings, United Kingdom
Publisher CryoLetters
Language eng
Subject 050202 Conservation and Biodiversity
Abstract Ex situ conservation of endangered plants is an important aim in order to preserve biodiversity of flora in threatened ecosystems. Among the biotechnological techniques which can be used, cryopreservation is emerging as a preferred option in many instances. This study describes a cryopreservation technique developed for shoot tips of the endangered species Centaurea ultreiae (Compositae) using a vitrification procedure. Basal medium (BM) for preculture and loading phases consisted of 1/2 MS basal salts with modified vitamins (3 μM thiamine). For preculturing shoot tips, BM with five osmotic treatments were investigated: 0.3 M sucrose ± 20 μM ABA, 0.6 M glycerol ± 20 μM ABA and 0.25 M sucrose + 0.25 M glycerol + 10 μM ABA. A loading solution treatment (BM with 2 M glycerol and 0.4 M sucrose) was applied prior to exposure of shoot tips to PVS2 and found to be indispensable to obtaining successful post-LN recovery. Highest (95.5%) regrowth of LN immersed shoot tips was obtained following incubation on BM + 0.3 M sucrose + 20 μM ABA or 0.25 M sucrose + 0.25 M glycerol + 10 μM ABA, with loading treatment and PVS2 exposure for 20 minutes at 0ºC.
Keyword Cryopreservation
Encapsulation
Endangered species
Ex situ conservation
Vitrification
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Thu, 03 Sep 2009, 09:20:30 EST by Mr Andrew Martlew on behalf of School of Pharmacy