In vitro model of vitamin D-3 (Cholecalciferol) synthesis by UV radiation: Dose-response relationships

Olds, William J., McKinley, Alex R., Moore, Michael R. and Kimlin, Michael G. (2008) In vitro model of vitamin D-3 (Cholecalciferol) synthesis by UV radiation: Dose-response relationships. Journal of Photochemistry and Photobiology B-biology, 93 2: 88-93. doi:10.1016/j.jphotobiol.2008.07.004


Author Olds, William J.
McKinley, Alex R.
Moore, Michael R.
Kimlin, Michael G.
Title In vitro model of vitamin D-3 (Cholecalciferol) synthesis by UV radiation: Dose-response relationships
Formatted title
In vitro model of vitamin D3 (Cholecalciferol) synthesis by UV radiation: Dose-response relationships
Journal name Journal of Photochemistry and Photobiology B-biology   Check publisher's open access policy
ISSN 1011-1344
1873-2682
Publication date 2008-11
Sub-type Article (original research)
DOI 10.1016/j.jphotobiol.2008.07.004
Volume 93
Issue 2
Start page 88
End page 93
Total pages 6
Place of publication Lausanne, Switzerland
Publisher Elsevier
Language eng
Abstract Vitamin D deficiency is a major health concern worldwide. Very little is understood regarding its production in the human body by exposure to UV radiation. In particular, we have no means of predicting how much vitamin D (cholecalciferol) will be produced in the skin after exposure to sunlight. Using a refined in vitro model, we found that there is a nonlinear relationship between UV dose and cholecalciferol synthesis. Two minimal erythemal doses (MED) of UV radiation produced 1.84 μg/mL of cholecalciferol whereas 4 MED produced 2.81 μg/mL. We also found that the production of cholecalciferol is restricted by the initial concentration of its precursor (7-dehydrocholesterol, 7-DHC). For example, using an initial concentration of 7-DHC of 102 μg/mL, the resultant cholecalciferol production was 1.05 μg/mL after receiving 4 MED exposure. Under the same exposure conditions, an initial concentration of 305 μg/mL yielded 2.81 g/mL of cholecalciferol. The data presented in this paper has important implications for humans, including: (1) increasing UV exposure does not result in a proportionate increase in the amount of cholecalciferol that is produced; and (2) the initial concentration of 7-DHC in the skin may impact the amount of cholecalciferol that can be synthesized. When translating these results to population groups, we will discuss how the sun exposure message needs to be carefully formulated to account for such considerations.
Keyword Vitamin D
Human skin
Ultraviolet
UV
Sun
Skin cancer
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 32 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 03 Sep 2009, 09:13:52 EST by Mr Andrew Martlew on behalf of National Res Centre For Environmental Toxicology