Cellulose hydrolysis by a methanogenic culture enriched from landfill waste in a semi-continuous reactor

Song, Hoyhak and Clarke, William P. (2009) Cellulose hydrolysis by a methanogenic culture enriched from landfill waste in a semi-continuous reactor. Bioresource Technology, 100 3: 1268-1273. doi:10.1016/j.biortech.2008.08.029


Author Song, Hoyhak
Clarke, William P.
Title Cellulose hydrolysis by a methanogenic culture enriched from landfill waste in a semi-continuous reactor
Journal name Bioresource Technology   Check publisher's open access policy
ISSN 0960-8524
1873-2976
Publication date 2009-02
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.biortech.2008.08.029
Volume 100
Issue 3
Start page 1268
End page 1273
Total pages 6
Editor A Pandey
C Larroche
S C Ricke
Place of publication Oxford, U.K.
Publisher Elsevier Sci
Language eng
Subject C1
850501 Biofuel (Biomass) Energy
090703 Environmental Technologies
100299 Environmental Biotechnology not elsewhere classified
Abstract This study investigates the hydrolysis of cellulose by a mixed culture enriched from landfill waste in a continuous reactor operated under prolonged residence times to accommodate methanogenic conditions. Chemostat studies of hydrolysis under balance methanogenic conditions are rarely reported, despite the importance of hydrolysis under these conditions in waste management and renewable energy industries. Continuous digestion was studied in a 1.25 l digester, fed with a 1% (w/v) slurry of 50 mu m cellulose in sterilized leachate drawn from a 220 l digester operated on a feedstock of mixed municipal solid waste. Unsterilized leachate was used as the inoculum. Stable and rapid hydrolytic conditions were established at residence time of 2.5, 3.5 and 5 d with a 1st order hydrolysis rate 0.45 +/- 0.07 d(-1) and high methane yields ranging from 57% to 62% of solubilised cellulose on a COD basis. Biomass yields were between 32% and 35% of solubilised cellulose on a COD basis, over three times that observed with fermentative cultures. This is attributed to the diversity of the microbial population which fully converted solubilised COD to methane, as evident by VFA yields of less than 8% on a COD basis.
Keyword Anaerobic digestion
Cellulose
Landfill organisms
Hydrolysis rate
Biomass yield
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Civil Engineering Publications
 
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Created: Thu, 03 Sep 2009, 09:12:56 EST by Mr Andrew Martlew on behalf of School of Civil Engineering