Comparative evaluation of methods commonly used to determine antimicrobial susceptibility to plant extracts and phenolic compounds

King, Thea, Dykes, Gary and Kristianti, Ruth (2008) Comparative evaluation of methods commonly used to determine antimicrobial susceptibility to plant extracts and phenolic compounds. Journal of AOAC International, 91 6: 1423-1429. doi:10.5555/jaoi.91.6.1423


Author King, Thea
Dykes, Gary
Kristianti, Ruth
Title Comparative evaluation of methods commonly used to determine antimicrobial susceptibility to plant extracts and phenolic compounds
Journal name Journal of AOAC International   Check publisher's open access policy
ISSN 1060-3271
1944-7922
Publication date 2008-11
Sub-type Article (original research)
DOI 10.5555/jaoi.91.6.1423
Volume 91
Issue 6
Start page 1423
End page 1429
Total pages 7
Editor Tien Milor
Place of publication Gaithersburg, MD, United States
Publisher A O A C International
Language eng
Formatted abstract
A comparison was made to evaluate the ability of the most commonly used qualitative agar diffusion methods and a quantitative broth dilution assay to determine the antimicrobial activity of a plant extract and a variety of phenolic compounds. A disc and well diffusion technique and a microtiter broth microdilution (MBM) assay were used as antimicrobial susceptibility tests of a plant extract and several phenolic compounds against 7 bacterial species. In both the well and disc diffusion assays, the level of reproducibility was poor and a linear or logarithmic relationship did not exist between inhibition zone size and the concentration of the agents. The MBM method produced the most consistent results and allowed the determination of the relative sensitivities of each species and the relative antimicrobial activities of each agent. This study demonstrated that when a diffusion method is used, multiple concentrations of the agent must be assayed to ensure that a relationship exists between the concentration of the agent and inhibition zone size. When a relationship does not exist, antimicrobial activity should be determined by a quantitative dilution technique.
Keyword Essential oils
Escherichia-coli
Screening methods
Basil
Flavonoids
Vegetables
Fruits
Thyme
Foods
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
ERA 2012 Admin Only
 
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Created: Thu, 03 Sep 2009, 09:06:15 EST by Mr Andrew Martlew on behalf of School of Land, Crop and Food Sciences